Any feedback?
Literature summary extracted from
- Kinetic studies on the inhibition of isopeptidase T by ubiquitin aldehyde (1996), Biochemistry, 35, 12893-12900.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.12 | ubiquitin | submicromolar concentrations activate while higher concentrations inhibit the enzyme, regulatory function, binding via 2 ubiquitin binding sites extending the active site | Oryctolagus cuniculus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.12 | ubiquitin | submicromolar concentrations activate while higher concentrations inhibit the enzyme, regulatory function, 2-step inhibition mechanism via 2 ubiquitin binding sites extending the active site | Oryctolagus cuniculus | |
| 3.4.19.12 | Ubiquitin aldehyde | inhibition in absence of ubiquitin, competitive to ubiquitin | Oryctolagus cuniculus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.12 | additional information | - |
additional information | kinetics | Oryctolagus cuniculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.12 | Oryctolagus cuniculus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.19.12 | reticulocyte | - |
Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.12 | polyubiquitin + H2O | - |
Oryctolagus cuniculus | ubiquitin | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.12 | 25 | - |
assay at | Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.12 | 7.8 | - |
assay at | Oryctolagus cuniculus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.12 | additional information | - |
additional information | inhibition kinetics | Oryctolagus cuniculus | |
| 3.4.19.12 | 0.0000001 | - |
ubiquitin-aldehyde | at 0.0006 mM ubiquitin, pH 7.8, 25°C | Oryctolagus cuniculus | |
| 3.4.19.12 | 0.0000023 | - |
ubiquitin-aldehyde | in absence of ubiquitin, pH 7.8, 25°C | Oryctolagus cuniculus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
