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Literature summary extracted from
- A human de-ubiquitinating enzyme with both isopeptidase and peptidase activities in vitro (1995), FEBS Lett., 359, 73-77.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.12 | peptides | containing either of the cleavage site sequence found in ubiquitin polymers, but not unrelated peptides | Homo sapiens |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.12 | 0.002 | - |
branched dimeric ubiquitin | pH 7.2, 37°C | Homo sapiens | |
| 3.4.19.12 | 0.015 | - |
head-to-tail dimeric ubiquitin | pH 7.2, 37°C | Homo sapiens |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.12 | 110000 | - |
analytical ultracentrifugation | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.12 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.19.12 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.19.12 | erythrocyte | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.12 | branched di-ubiquitin + H2O | recombinant substrate from expression in Escherichia coli | Homo sapiens | ubiquitin | - |
? | |
| 3.4.19.12 | branched dimeric ubiquitin + H2O | - |
Homo sapiens | ubiquitin | - |
? | |
| 3.4.19.12 | head-to-tail dimeric ubiquitin + H2O | - |
Homo sapiens | ubiquitin | - |
? | |
| 3.4.19.12 | head-to-tail polyubiquitin + H2O | recombinant substrate from expression in Escherichia coli | Homo sapiens | ubiquitin | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.19.12 | monomer | 1 * 110000, SDS-PAGE, monomeric under reducing conditions | Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.12 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.12 | 7.2 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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