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Literature summary extracted from
- Purification and characterization of rat liver microsomal fatty acid ethyl and 2-chloroethyl ester synthase and their relationship with carboxylesterase (pI 6.1) (1997), Chem. Res. Toxicol., 10, 211-218.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.67 | diisopropylfluorophosphate | 180 kDa high molecular weight protein from liver microsomes | Rattus norvegicus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.67 | 0.8 | - |
oleate | pH 7.4, 37°C, 180 kDa high molecular weight protein from liver microsomes | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.67 | microsome | 180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction in liver microsomes with FAEES activity | Rattus norvegicus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.67 | 60000 | - |
low molecular weight minor protein fraction in liver microsomes with FAEES activity, gel filtration | Rattus norvegicus |
| 3.1.1.67 | 180000 | - |
high molecular weight major protein fraction in liver microsomes with FAEES activity, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.67 | additional information | Rattus norvegicus | 180 kDa high molecular weight protein from liver microsomes, involved in the biosynthesis of long-chain fatty acid esters of xenobiotic alcohols | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.67 | Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.67 | copurification of FAEES, 2-chloroethyl ester synthase and carboxylesterase activity during each step of purification, FAEES activity: 30.9fold | Rattus norvegicus |
| 3.1.1.67 | from liver microsomes, 180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.67 | liver | 180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction in liver microsomes with FAEES activity | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.67 | 0.0335 | - |
pH 7.4, 37°C, 180 kDa high molecular weight protein from liver microsomes, FAEES activity | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.67 | cholesteryl oleate + H2O | 180 kDa high molecular weight protein, only hydrolysis, no synthetic activity for cholesterol oleate | Rattus norvegicus | oleate + cholesterol | - |
ir | |
| 3.1.1.67 | fatty acid + 2-chloroethanol | long-chain fatty acids, 180 kDa high molecular weight protein | Rattus norvegicus | fatty acyl 2-chloroethyl ester + H2O | - |
? | |
| 3.1.1.67 | fatty acid + ethanol | long-chain fatty acids | Rattus norvegicus | fatty acyl ethyl ester + H2O | - |
? | |
| 3.1.1.67 | additional information | FAEES, 2-chloroethyl ester synthase and carboxylesterase activities are expressed by the same 180 kDa trimeric protein in liver microsomes, structural similarity with rat hepatic microsomal carboxylesterase | Rattus norvegicus | ? | - |
? | |
| 3.1.1.67 | additional information | 180 kDa high molecular weight protein from liver microsomes, involved in the biosynthesis of long-chain fatty acid esters of xenobiotic alcohols | Rattus norvegicus | ? | - |
? | |
| 3.1.1.67 | oleate + 2-chloroethanol | 180 kDa high molecular weight protein | Rattus norvegicus | 2-chloroethyl oleate + H2O | - |
? | |
| 3.1.1.67 | oleate + ethanol | - |
Rattus norvegicus | ethyl oleate + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.67 | trimer | 3 * 60000, high molecular weight major protein fraction, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.67 | 37 | - |
assay at | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.67 | 0.285 | - |
oleate | pH 7.4, 37°C, 180 kDa high molecular weight protein from liver microsomes | Rattus norvegicus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.67 | 7.4 | - |
assay at | Rattus norvegicus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.1.67 | Rattus norvegicus | 180 kDa high molecular weight protein from liver microsomes, isoelectric focusing | - |
6.1 |
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