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Literature summary extracted from

  • Kaphalia, B.S.; Ansari, G.A.S.
    Purification and characterization of rat hepatic microsomal low molecular weight fatty acid ethyl ester synthase and its relationship to carboxylesterases (2001), J. Biochem. Mol. Toxicol., 15, 165-171.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.67 microsome 180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction in liver microsomes with FAEES activity Rattus norvegicus
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Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.67 60000
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 low molecular weight minor protein fraction in liver microsomes with FAEES activity, gel filtration Rattus norvegicus
3.1.1.67 180000
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 high molecular weight major protein fraction in liver microsomes with FAEES activity, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.67 Rattus norvegicus
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-
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.67 5 distinct 60 kDa low molecular weight proteins, 10.2-32.5fold Rattus norvegicus
3.1.1.67 from liver microsomes, 180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.67 liver 180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction in liver microsomes with FAEES activity Rattus norvegicus
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.67 0.0111
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 60 kDa low molecular weight protein from liver microsomes with pI 5.3 Rattus norvegicus
3.1.1.67 0.0115
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 60 kDa low molecular weight protein from liver microsomes with pI 6.5 Rattus norvegicus
3.1.1.67 0.0125
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 60 kDa low molecular weight protein from liver microsomes with pI 5.0 Rattus norvegicus
3.1.1.67 0.0133
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 60 kDa low molecular weight protein from liver microsomes with pI 5.6 Rattus norvegicus
3.1.1.67 0.035
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 60 kDa low molecular weight protein from liver microsomes with pI 5.8 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.67 fatty acid + ethanol
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 Rattus norvegicus fatty acyl ethyl ester + H2O
-
 ?
3.1.1.67 additional information structural and functional relationship to hepatic microsomal carboxylesterases, low molecular weight FAEE synthase activity most probably is expressed by various isozymes of hepatic microsomal carboxylesterases, which are also involved in the biotransformation of xenobiotic alcohols and amines, the LMW protein fraction also exhibits fatty acid 2-chloroethyl ester synthase and p-nitrophenyl acetate-hydrolyzing activity Rattus norvegicus ?
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 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.67 monomer 1 * 60000, low molecular weight minor protein fraction, SDS-PAGE Rattus norvegicus
3.1.1.67 trimer 3 * 60000, high molecular weight major protein fraction, SDS-PAGE Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.67 Rattus norvegicus one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
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 5
3.1.1.67 Rattus norvegicus one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
-
 5.3
3.1.1.67 Rattus norvegicus one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing 5.9 5.6
3.1.1.67 Rattus norvegicus one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
-
 5.8
3.1.1.67 Rattus norvegicus one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
-
 6.5