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Literature summary extracted from

  • Tsujita, T.; Okuda, H.
    Fatty acid ethyl ester synthase in rat adipose tissue and its relationship to carboxylesterase (1992), J. Biol. Chem., 267, 23489-23494.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.67 diisopropylfluorophosphate 0.001 mM, 50% inhibition after 10 min preincubation at 37°C Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.67 0.71
-
ethanol cosubstrate oleate Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.67 60000
-
x * 60000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.67 fatty acid + ethanol Rattus norvegicus enzyme contributes to the nonoxidative ethanol metabolism in various organs fatty acyl ethyl ester + H2O
-
?
3.1.1.67 fatty acid + ethanol Rattus norvegicus production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects fatty acyl ethyl ester + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.67 Rattus norvegicus
-
Wistar rats weighing 300-400 g
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.67 from adipose tissue, 2 enzyme forms Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.67 adipose tissue
-
Rattus norvegicus
-
3.1.1.67 liver
-
Rattus norvegicus
-
3.1.1.67 lung
-
Rattus norvegicus
-
3.1.1.67 additional information not: kidney Rattus norvegicus
-
3.1.1.67 testis
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.67 additional information
-
-
Rattus norvegicus
3.1.1.67 0.015
-
pH 7, 37°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.67 fatty acid + ethanol substrate specificity Rattus norvegicus fatty acyl ethyl ester + H2O
-
?
3.1.1.67 fatty acid + ethanol enzyme contributes to the nonoxidative ethanol metabolism in various organs Rattus norvegicus fatty acyl ethyl ester + H2O
-
?
3.1.1.67 fatty acid + ethanol production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects Rattus norvegicus fatty acyl ethyl ester + H2O
-
?
3.1.1.67 monoacylglycerol + H2O hydrolysis, susceptibilities of substrates increase with decreasing acyl chain length of the fatty acid moiety Rattus norvegicus fatty acid + glycerol
-
?
3.1.1.67 additional information FAEES is associated with carboxylesterase in adipose tissue Rattus norvegicus ?
-
?
3.1.1.67 oleate + ethanol ethyl oleate-hydrolyzing activity is about one-eighth of the synthesizing activity Rattus norvegicus ethyl oleate + H2O
-
r
3.1.1.67 triacylglycerol + H2O hydrolysis, susceptibilities of substrates increase with decreasing acyl chain length of the fatty acid moiety Rattus norvegicus ?
-
?
3.1.1.67 triolein + H2O
-
Rattus norvegicus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.67 ? x * 60000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.67 37
-
assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.67 7
-
optimum and assay at Rattus norvegicus