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Literature summary extracted from
- Thermodynamic bases for fatty acid ethyl ester synthase catalyzed esterification of free fatty acid with ethanol and accumulation of fatty acid ethyl esters (1984), Biochemistry, 23, 4082-4087.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.67 | additional information | - |
additional information | thermodynamic parameters relevant to the kinetics and equilibria of the reaction | Oryctolagus cuniculus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.67 | cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.67 | Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.67 | - |
Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.67 | heart | myocardium | Oryctolagus cuniculus | - |
| 3.1.1.67 | myocardium | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.67 | additional information | - |
- |
Oryctolagus cuniculus |
| 3.1.1.67 | 0.0003 | - |
pH 7.2, 37°C | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.67 | fatty acid + ethanol | - |
Oryctolagus cuniculus | fatty acyl ethyl ester + H2O | - |
? |  |
| 3.1.1.67 | oleate + ethanol | equilibrium data | Oryctolagus cuniculus | ethyl oleate + H2O | product accumulates in an organic phase | ? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.67 | 37 | - |
assay at | Oryctolagus cuniculus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.67 | 0.024 | - |
oleate | pH 7.2, 20°C, formation of ethyl oleate | Oryctolagus cuniculus | |
| 3.1.1.67 | 0.04 | - |
oleate | pH 7.2, 30°C, formation of ethyl oleate | Oryctolagus cuniculus | |
| 3.1.1.67 | 0.06 | - |
oleate | pH 7.2, 37°C, formation of ethyl oleate | Oryctolagus cuniculus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.67 | 7.2 | - |
assay at | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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