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Literature summary extracted from
- Partial purification and product characterization of fatty acid ethyl ester synthases in rabbit myocardium (1984), Biochim. Biophys. Acta, 798, 144-148.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.67 | 650 | - |
ethanol | pH 7.2, 37°C, minor cytosolic enzyme activity | Oryctolagus cuniculus |  |
| 3.1.1.67 | 750 | - |
ethanol | pH 7.2, 37°C, major cytosolic enzyme activity | Oryctolagus cuniculus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.67 | cytosol | - |
Oryctolagus cuniculus | 5829 | - |
| 3.1.1.67 | soluble | - |
Oryctolagus cuniculus | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.67 | fatty acid + ethanol | Oryctolagus cuniculus | myocardial nonoxidative ethanol metabolism, metabolism of fatty acids independent of coenzyme A | fatty acyl ethyl ester + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.67 | Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.67 | partial, separation of 2 cytosolic enzymes: 26fold and 30fold | Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.67 | heart | ventricular myocardium | Oryctolagus cuniculus | - |
| 3.1.1.67 | myocardium | ventricular | Oryctolagus cuniculus | - |
| 3.1.1.67 | myocardium | 2 enzymes accounting for 19% and 81% of the total fatty acid ethyl ester synthetic capability | Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.67 | fatty acid + ethanol | - |
Oryctolagus cuniculus | fatty acyl ethyl ester + H2O | - |
? | |
| 3.1.1.67 | fatty acid + ethanol | myocardial nonoxidative ethanol metabolism, metabolism of fatty acids independent of coenzyme A | Oryctolagus cuniculus | fatty acyl ethyl ester + H2O | - |
? | |
| 3.1.1.67 | oleate + ethanol | - |
Oryctolagus cuniculus | ethyl oleate + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.67 | 37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.67 | 7.2 | - |
assay at | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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