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Literature summary extracted from

  • Jacob, U.; Mack, M.; Clausen, T.; Huber, R.; Buckel, W.; Messerschmidt, A.
    Glutaconate CoA-transferase from Acidaminococcus fermentans: the crystal structure reveals homology with other CoA-transferases (1997), Structure, 5, 415-426.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.12 GCT expression in Escherichia coli Acidaminococcus fermentans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.3.12 recombinant GCT, expressed in Escherichia coli, sitting-drop vapour diffusion method Acidaminococcus fermentans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.12 29000
-
 alpha4beta4, 4 * 35000 + 4 * 29000, structure and association of the subunits A and B Acidaminococcus fermentans
2.8.3.12 35000
-
 alpha4beta4, 4 * 35000 + 4 * 29000, structure and association of the subunits A and B Acidaminococcus fermentans
2.8.3.12 260000
-
 heterooctamer GCT Acidaminococcus fermentans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.12 acetyl-CoA + (R)-2-hydroxyglutarate Acidaminococcus fermentans in the course of glutamate fermentation acetate + (R)-2-hydroxyglutaryl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.12 Acidaminococcus fermentans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.12 recombinant GCT, expressed in Escherichia coli Acidaminococcus fermentans

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.3.12 acetyl-CoA + (E)-glutaconate = acetate + glutaconyl-1-CoA ping-pong mechanism Acidaminococcus fermentans
a
2.8.3.12 acetyl-CoA + (E)-glutaconate = acetate + glutaconyl-1-CoA reaction mechanism Acidaminococcus fermentans
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.12 acetyl-CoA + (E)-glutaconate
-
 Acidaminococcus fermentans acetate + glutaconyl-1-CoA
-
 r
2.8.3.12 acetyl-CoA + (R)-2-hydroxyglutarate
-
 Acidaminococcus fermentans acetate + (R)-2-hydroxyglutaryl-1-CoA
-
 r
2.8.3.12 acetyl-CoA + (R)-2-hydroxyglutarate in the course of glutamate fermentation Acidaminococcus fermentans acetate + (R)-2-hydroxyglutaryl-CoA
-
 r
2.8.3.12 additional information GCT structure, the active site is located at the interface of subunits A and B and is formed by loops of both subunits Acidaminococcus fermentans ?
-
 ?
2.8.3.12 additional information catalytic residue is E-54 of subunit B, catalytic mechanism Acidaminococcus fermentans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.3.12 octamer alpha4beta4, 4 * 35000 + 4 * 29000, structure and association of the subunits A and B Acidaminococcus fermentans