EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.3.12 | 0.035 | - |
(R)-2-hydroxyglutaryl-1-CoA | pH 7, 37°C, cosubstrate acetate | Acidaminococcus fermentans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.3.12 | Acidaminococcus fermentans | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.12 | acetyl-CoA + (E)-glutaconate | - |
Acidaminococcus fermentans | acetate + glutaconyl-1-CoA | only 1-isomer is formed | ? | |
2.8.3.12 | acetyl-CoA + (R)-2-hydroxyglutarate | - |
Acidaminococcus fermentans | acetate + (R)-2-hydroxyglutaryl-1-CoA | in vitro formation of both isomers: (R)-2-hydroxyglutaryl-1-CoA and (R)-2-hydroxyglutaryl-5-CoA, the 1-isomer is kinetically favoured, the 5-isomer is thermodynamically favoured | r | |
2.8.3.12 | acetyl-CoA + (R)-2-hydroxyglutarate | - |
Acidaminococcus fermentans | acetate + (R)-2-hydroxyglutaryl-1-CoA | product in vivo, 5-isomer only occurs in vitro, (R)-2-hydroxyglutaryl-1-CoA is an excellent substrate in the reverse reaction | r | |
2.8.3.12 | glutaconyl-CoA + (R)-2-hydroxyglutarate | - |
Acidaminococcus fermentans | glutaconate + (R)-2-hydroxyglutaryl-CoA | formation of the 5-isomer, not of the 1-isomer | ? | |
2.8.3.12 | additional information | no substrate: lactone-CoA | Acidaminococcus fermentans | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.3.12 | 37 | - |
assay at | Acidaminococcus fermentans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.3.12 | 7 | - |
assay at | Acidaminococcus fermentans |