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Literature summary extracted from

  • Mikami, T.; Mizumoto, S.; Kago, N.; Kitagawa, H.; Sugahara, K.
    Specificities of three distinct human chondroitin/dermatan N-acetylgalactosamine 4-O-sulfotransferases demonstrated using partially desulfated dermatan sulfate as an acceptor. Implication of differential roles in dermatan sulfate biosynthesis (2003), J. Biol. Chem., 278, 36115-36127.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.5 expression as N-terminally truncated enzyme, lacking the first 57 amino acids, in COS-1 cells Homo sapiens
2.8.2.5 expression as N-terminally truncated enzyme, lacking the first 76 amino acids, in COS-1 cells Homo sapiens
2.8.2.35 expression in Cos-1 cells Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.5 Homo sapiens Q8NET6 isozyme C4ST3
-
2.8.2.5 Homo sapiens Q9NPF2 isozyme C4ST1
-
2.8.2.5 Homo sapiens Q9NRB3 isozyme C4ST2
-
2.8.2.35 Homo sapiens Q8NCH0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.5 3'-phosphoadenylylsulfate + chondroitin
-
 Homo sapiens adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
 ?
2.8.2.5 3'-phosphoadenylylsulfate + chondroitin isozyme C4ST1, best substrate Homo sapiens adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
 ?
2.8.2.5 3'-phosphoadenylylsulfate + chondroitin sulfate C low activity Homo sapiens adenosine 3',5'-bisphosphate + chondroitin sulfate C 4'-sulfate
-
 ?
2.8.2.5 3'-phosphoadenylylsulfate + chondroitin sulfate D low activity Homo sapiens adenosine 3',5'-bisphosphate + chondroitin sulfate D 4'-sulfate
-
 ?
2.8.2.5 3'-phosphoadenylylsulfate + desulfated chondroitin sulfate A low activity Homo sapiens adenosine 3',5'-bisphosphate + chondroitin sulfate A
-
 ?
2.8.2.5 3'-phosphoadenylylsulfate + desulfated chondroitin sulfate B low activity Homo sapiens adenosine 3',5'-bisphosphate + chondroitin sulfate B
-
 ?
2.8.2.5 3'-phosphoadenylylsulfate + partially desulfated dermatan sulfate
-
 Homo sapiens ?
-
 ?
2.8.2.5 3'-phosphoadenylylsulfate + partially desulfated dermatan sulfate isozyme C4ST2, best substrate Homo sapiens ?
-
 ?
2.8.2.5 3'-phosphoadenylylsulfate + partially desulfated dermatan sulfate isozyme C4ST3, very low activity Homo sapiens ?
-
 ?
2.8.2.5 additional information product structure analysis Homo sapiens ?
-
 ?
2.8.2.5 additional information chondroitin sulfate E is no substrate Homo sapiens ?
-
 ?
2.8.2.35 3'-phospho-5'-adenylyl sulfate + [dermatan]-N-acetylgalactosamine most efficiently utilizes GalNAc residues located not only in the sequence -IdoUA-GalNAc-IdoUA- but also in -GlcUA-Gal-NAc-IdoUA- and -IdoUA-GalNAc-GlcUA-. The isolated oligosaccharide structures suggest that 4-O-sulfation promotes subsequent 4-O-sulfation of GalNAc in the neighboring disaccharide unit Homo sapiens adenosine 3'-5'-bisphosphate + [dermatan]-4-O-sulfo-N-acetylgalactosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.35 D4ST-1
-
 Homo sapiens
2.8.2.35 dermatan 4-O-sulfotransferase-1
-
 Homo sapiens
2.8.2.35 dermatan N-acetylgalactosamine 4-O-sulfotransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.5 28
-
 assay at Homo sapiens
2.8.2.5 37
-
 assay at Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.8.2.5 additional information
-
 isozyme C4ST3 shows very poor activity with chondroitin and partially desulfated dermatan sulfate as substrates at 37°C compared to 28°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.5 6.8
-
 assay at Homo sapiens