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Literature summary extracted from

  • Yamauchi, S.; Hirahara, Y.; Usui, H.; Takeda, Y.; Hoshino, M.; Fukuta, M.; Kimura, J.H.; Habuchi, O.
    Purification and characterization of chondroitin 4-sulfotransferase from the culture medium of a rat chondrosarcoma cell line (1999), J. Biol. Chem., 274, 2456-2463.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.5 2-mercaptoethanol stimulates Rattus norvegicus
2.8.2.5 dithiothreitol activation of the purified enzyme Rattus norvegicus
2.8.2.5 glutathione reduced glutathione stimulates Rattus norvegicus
2.8.2.5 protamine stimulates Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.5 0.00027
-
 3'-phosphoadenylylsulfate pH 6.8, 37°C, in presence of protamine Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.5 extracellular enzyme is released into the cell culture medium Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.5 Ba2+ stimulates Rattus norvegicus
2.8.2.5 Ca2+ highest stimulatory effect, optimal concentration: 5 mM Rattus norvegicus
2.8.2.5 Fe2+ stimulates Rattus norvegicus
2.8.2.5 Mg2+ slightly stimulating Rattus norvegicus
2.8.2.5 Mn2+ stimulates Rattus norvegicus
2.8.2.5 Sr2+ stimulates Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.5 35000
-
 1 * 35000, deglycosylated protein, SDS-PAGE Rattus norvegicus
2.8.2.5 50000
-
 about, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.5 3'-phosphoadenylylsulfate + chondroitin Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.5 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.8.2.5 glycoprotein enzyme contains N-linked oligosaccharides Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.5 1920fold, to homogeneity, from the serum-free cell culture medium Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.5 cell culture chondrosarcoma cell line Rattus norvegicus
-
2.8.2.5 chondrosarcoma cell extracellular, secretion to the medium Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.5 0.000006
-
 purified enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.8.2.5 -20°C, purified enzyme, loss of 80% activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.5 3'-phosphoadenylylsulfate + chondroitin
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
 ?
2.8.2.5 3'-phosphoadenylylsulfate + chondroitin sulfate C low activity Rattus norvegicus adenosine 3',5'-bisphosphate + chondroitin sulfate C 4'-sulfate
-
 ?
2.8.2.5 3'-phosphoadenylylsulfate + desulfated chondroitin sulfate A low activity Rattus norvegicus adenosine 3',5'-bisphosphate + chondroitin sulfate A
-
 ?
2.8.2.5 3'-phosphoadenylylsulfate + desulfated dermatan sulfate
-
 Rattus norvegicus ?
-
 ?
2.8.2.5 additional information no activity with dermatan sulfate, keratan sulfate Rattus norvegicus ?
-
 ?
2.8.2.5 additional information chondroitin sulfate E, heparan sulfate, and completely desulfated N-resulfated heparin are no acceptor substrates Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.5 monomer 1 * 35000, deglycosylated protein, SDS-PAGE Rattus norvegicus
2.8.2.5 monomer 1 * 50000-54000, nonreducing conditions, SDS-PAGE Rattus norvegicus
2.8.2.5 monomer 1 * 60000-64000, reducing conditions, SDS-PAGE Rattus norvegicus
2.8.2.5 More the deglycosylated enzyme forms dimers in solution of 70 kDa Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.8.2.5 C4ST
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.5 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.5 7.2
-
-
 Rattus norvegicus