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Literature summary extracted from

  • Petrotchenko, E.V.; Doerflein, M.E.; Kakuta, Y.; Pedersen, L.C.; Negishi, M.
    Substrate gating confers steroid specificity to estrogen sulfotransferase (1999), J. Biol. Chem., 274, 30019-30022.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.4 EST cloned and expressed in Escherichia coli Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.4 C84F site-directed mutagenesis Mus musculus
2.8.2.4 C84Y site-directed mutagenesis Mus musculus
2.8.2.4 F142A site-directed mutagenesis Mus musculus
2.8.2.4 F142L site-directed mutagenesis Mus musculus
2.8.2.4 F24A site-directed mutagenesis Mus musculus
2.8.2.4 I146A site-directed mutagenesis Mus musculus
2.8.2.4 M247G site-directed mutagenesis Mus musculus
2.8.2.4 N86A site-directed mutagenesis Mus musculus
2.8.2.4 R23A site-directed mutagenesis Mus musculus
2.8.2.4 S148A site-directed mutagenesis Mus musculus
2.8.2.4 Y149F site-directed mutagenesis Mus musculus
2.8.2.4 Y240F site-directed mutagenesis Mus musculus
2.8.2.4 Y81A site-directed mutagenesis Mus musculus
2.8.2.4 Y81F site-directed mutagenesis Mus musculus
2.8.2.4 Y81L site-directed mutagenesis Mus musculus
2.8.2.4 Y81L/F142L site-directed mutagenesis Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.4 0.000025
-
 estradiol pH 8.0, 37°C wild-type Mus musculus
2.8.2.4 0.000032
-
 estradiol pH 8.0, 37°C mutant Y81F Mus musculus
2.8.2.4 0.000092
-
 estradiol pH 8.0, 37°C mutant Y81L Mus musculus
2.8.2.4 0.000235
-
 estradiol pH 8.0, 37°C mutant Y81A Mus musculus
2.8.2.4 0.000735
-
 estradiol pH 8.0, 37°C mutant Y81L/F142L Mus musculus
2.8.2.4 0.000812
-
 estradiol pH 8.0, 37°C mutant F142L Mus musculus
2.8.2.4 0.000868
-
 androstenediol pH 8.0, 37°C mutant Y81L Mus musculus
2.8.2.4 0.00125
-
 estradiol pH 8.0, 37°C mutant F142A Mus musculus
2.8.2.4 0.0029
-
 androstenediol pH 8.0, 37°C mutant F142L Mus musculus
2.8.2.4 0.003224
-
 androstenediol pH 8.0, 37°C wild-type Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.4 3'-phosphoadenylylsulfate + estradiol Mus musculus
-
 adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.4 Mus musculus
-
 mouse
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.4 recombinant enzyme Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.4 3'-phosphoadenylylsulfate + androstenediol wild-type EST exhibits little ability to sulfate AD Mus musculus adenosine 3',5'-bisphosphate + androstenediol sulfate
-
 r
2.8.2.4 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Mus musculus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r
2.8.2.4 3'-phosphoadenylylsulfate + estradiol
-
 Mus musculus adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
 r