BRENDA - Enzyme Database show

Isolation and expression of an isoform of rat estrogen sulfotransferase

Falany, J.L.; Krasnykh, V.; Mikheeva, G.; Falany, C.N.; J. Steroid Biochem. Mol. Biol. 52, 35-44 (1995)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.8.2.4
isoform rEST-6 cloned and expressed in Escherichia coli
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.4
17beta-estradiol
substrate inhibition
Rattus norvegicus
2.8.2.4
estrone
substrate inhibition
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.2.4
cytosol
-
Rattus norvegicus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.8.2.4
additional information
varying the Mg2+ concentration from 0-10 mM in the beta-estradiol sulfation reaction does not affect the sulfation activity of isoform rEST-6
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.4
35300
-
calculated from putative amino acid sequence
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.2.4
Rattus norvegicus
-
rat
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.2.4
isoform rEST-6
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + 17beta-estradiol
isoform rEST-6
645890
Rattus norvegicus
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
-
-
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
isoform rEST-6
645890
Rattus norvegicus
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645890
Rattus norvegicus
r
2.8.2.4
additional information
isoform rEST-6 selectively expressed in male rats sulfates estrogens, but dehydroepiandrosterone, pregnenolone, cortisol or testosterone are no substrates
645890
Rattus norvegicus
?
-
-
-
-
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.2.4
isoform rEST-6 cloned and expressed in Escherichia coli
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.4
17beta-estradiol
substrate inhibition
Rattus norvegicus
2.8.2.4
estrone
substrate inhibition
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.2.4
cytosol
-
Rattus norvegicus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.8.2.4
additional information
varying the Mg2+ concentration from 0-10 mM in the beta-estradiol sulfation reaction does not affect the sulfation activity of isoform rEST-6
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.4
35300
-
calculated from putative amino acid sequence
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.2.4
isoform rEST-6
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + 17beta-estradiol
isoform rEST-6
645890
Rattus norvegicus
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
-
-
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
isoform rEST-6
645890
Rattus norvegicus
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645890
Rattus norvegicus
r
2.8.2.4
additional information
isoform rEST-6 selectively expressed in male rats sulfates estrogens, but dehydroepiandrosterone, pregnenolone, cortisol or testosterone are no substrates
645890
Rattus norvegicus
?
-
-
-
-