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Literature summary extracted from

  • Hobkirk, R.; Glasier, M.A.; Brown, L.Y.
    Purification and some characteristics of an oestrogen sulphotransferase from guinea pig adrenal gland and its non-identity with adrenal pregnenolone sulphotransferase (1990), Biochem. J., 268, 759-764.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.4 cytosol
-
 Cavia porcellus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.4 67000
-
 gel filtration Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.4 3'-phosphoadenylylsulfate + estradiol Cavia porcellus
-
 adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
 r
2.8.2.4 3'-phosphoadenylylsulfate + estradiol Cavia porcellus Hartley
-
 adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
 r
2.8.2.4 3'-phosphoadenylylsulfate + estrone Cavia porcellus
-
 adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
 r
2.8.2.4 3'-phosphoadenylylsulfate + estrone Cavia porcellus Hartley
-
 adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.4 Cavia porcellus
-
 guinea pig
-
2.8.2.4 Cavia porcellus
-
 english shorthair
-
2.8.2.4 Cavia porcellus
-
 Hartley
-
2.8.2.4 Cavia porcellus Hartley
-
 Hartley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.4
-
 Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.4 adrenal gland
-
 Cavia porcellus
-
2.8.2.4 ovary
-
 Cavia porcellus
-
2.8.2.4 testis
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.4 0.0274
-
-
 Cavia porcellus

Storage Stability

EC Number Storage Stability Organism
2.8.2.4 -20°C, freshly prepared adrenal cytosol loses no estrogen sulfotransferase activity during storage for periods of up to 5 months Cavia porcellus
2.8.2.4 -20°C, partially purified enzyme may be stored for 2-3 weeks without loss of activity Cavia porcellus
2.8.2.4 0°C, fresh cytosol retains 85% of activity after 20 days, 50% after 50 days Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.4 3'-phosphoadenylylsulfate + estradiol
-
 Cavia porcellus adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
 r
2.8.2.4 3'-phosphoadenylylsulfate + estradiol
-
 Cavia porcellus Hartley adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
 r
2.8.2.4 3'-phosphoadenylylsulfate + estrone
-
 Cavia porcellus adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
 r
2.8.2.4 3'-phosphoadenylylsulfate + estrone
-
 Cavia porcellus Hartley adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
 r

Subunits

EC Number Subunits Comment Organism
2.8.2.4 dimer 2 * 34000-36000, SDS-PAGE Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.4 8
-
-
 Cavia porcellus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.2.4 Cavia porcellus 2 forms of the enzyme, FPLC chromatofocusing in a pH gradient of 4.0-7.1
-
 5.5
2.8.2.4 Cavia porcellus 2 forms of the enzyme, FPLC chromatofocusing in a pH gradient of 4.0-7.1
-
 6.1