BRENDA - Enzyme Database show

Purification and some characteristics of an oestrogen sulphotransferase from guinea pig adrenal gland and its non-identity with adrenal pregnenolone sulphotransferase

Hobkirk, R.; Glasier, M.A.; Brown, L.Y.; Biochem. J. 268, 759-764 (1990)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.2.4
cytosol
-
Cavia porcellus
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.4
67000
-
gel filtration
Cavia porcellus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
Cavia porcellus
-
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
Cavia porcellus
r
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
Cavia porcellus Hartley
-
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
Cavia porcellus Hartley
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Cavia porcellus
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Cavia porcellus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Cavia porcellus Hartley
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Cavia porcellus Hartley
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.2.4
Cavia porcellus
-
english shorthair; guinea pig; Hartley
-
2.8.2.4
Cavia porcellus Hartley
-
Hartley
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.2.4
-
Cavia porcellus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
adrenal gland
-
Cavia porcellus
-
2.8.2.4
ovary
-
Cavia porcellus
-
2.8.2.4
testis
-
Cavia porcellus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.2.4
0.0274
-
-
Cavia porcellus
Storage Stability
EC Number
Storage Stability
Organism
2.8.2.4
-20°C, freshly prepared adrenal cytosol loses no estrogen sulfotransferase activity during storage for periods of up to 5 months
Cavia porcellus
2.8.2.4
-20°C, partially purified enzyme may be stored for 2-3 weeks without loss of activity
Cavia porcellus
2.8.2.4
0°C, fresh cytosol retains 85% of activity after 20 days, 50% after 50 days
Cavia porcellus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
-
645879
Cavia porcellus
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
645879
Cavia porcellus
r
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
-
645879
Cavia porcellus Hartley
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
645879
Cavia porcellus Hartley
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645879
Cavia porcellus
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645879
Cavia porcellus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645879
Cavia porcellus Hartley
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645879
Cavia porcellus Hartley
r
Subunits
EC Number
Subunits
Commentary
Organism
2.8.2.4
dimer
2 * 34000-36000, SDS-PAGE
Cavia porcellus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.2.4
8
-
-
Cavia porcellus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.8.2.4
Cavia porcellus
2 forms of the enzyme, FPLC chromatofocusing in a pH gradient of 4.0-7.1
-
5.5
2.8.2.4
Cavia porcellus
2 forms of the enzyme, FPLC chromatofocusing in a pH gradient of 4.0-7.1
-
6.1
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.2.4
cytosol
-
Cavia porcellus
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.4
67000
-
gel filtration
Cavia porcellus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
Cavia porcellus
-
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
Cavia porcellus
r
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
Cavia porcellus Hartley
-
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
Cavia porcellus Hartley
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Cavia porcellus
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Cavia porcellus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Cavia porcellus Hartley
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Cavia porcellus Hartley
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.2.4
-
Cavia porcellus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
adrenal gland
-
Cavia porcellus
-
2.8.2.4
ovary
-
Cavia porcellus
-
2.8.2.4
testis
-
Cavia porcellus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.2.4
0.0274
-
-
Cavia porcellus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.8.2.4
-20°C, freshly prepared adrenal cytosol loses no estrogen sulfotransferase activity during storage for periods of up to 5 months
Cavia porcellus
2.8.2.4
-20°C, partially purified enzyme may be stored for 2-3 weeks without loss of activity
Cavia porcellus
2.8.2.4
0°C, fresh cytosol retains 85% of activity after 20 days, 50% after 50 days
Cavia porcellus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
-
645879
Cavia porcellus
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
645879
Cavia porcellus
r
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
-
645879
Cavia porcellus Hartley
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
645879
Cavia porcellus Hartley
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645879
Cavia porcellus
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645879
Cavia porcellus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645879
Cavia porcellus Hartley
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645879
Cavia porcellus Hartley
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.8.2.4
dimer
2 * 34000-36000, SDS-PAGE
Cavia porcellus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.2.4
8
-
-
Cavia porcellus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.8.2.4
Cavia porcellus
2 forms of the enzyme, FPLC chromatofocusing in a pH gradient of 4.0-7.1
-
5.5
2.8.2.4
Cavia porcellus
2 forms of the enzyme, FPLC chromatofocusing in a pH gradient of 4.0-7.1
-
6.1