BRENDA - Enzyme Database show

Characteristics and behavior during partial purification of estrogen sulfotransferase of guinea pig liver and chorion

Dick, C.M.; Hobkirk, R.; Biochim. Biophys. Acta 925, 362-370 (1987)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.2.4
monothiolglycerol
activity is stimulated 10fold by 25 mM
Cavia porcellus
General Stability
EC Number
General Stability
Organism
2.8.2.4
not stabilized by 20% glycerol or 25 mM NaCl
Cavia porcellus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.4
ZnCl2
inhibition of liver isoenzyme at 15 mM
Cavia porcellus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.2.4
cytosol
-
Cavia porcellus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.8.2.4
Ca2+
activity is stimulated 2fold by 15 mM CaCl2
Cavia porcellus
2.8.2.4
Mg2+
activity is stimulated 2fold by 15 mM MgCl2
Cavia porcellus
2.8.2.4
Zn2+
weakly stimulatory at 2.5 mM ZnCl2
Cavia porcellus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.4
50000
-
chorion enzyme, gel filtration
Cavia porcellus
2.8.2.4
52300
-
liver enzyme, gel filtration
Cavia porcellus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
Cavia porcellus
-
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
Cavia porcellus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Cavia porcellus
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Cavia porcellus
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.2.4
Cavia porcellus
-
english shorthair; guinea pig
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.2.4
partial, liver and chorion isoenzymes
Cavia porcellus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
chorion
-
Cavia porcellus
-
2.8.2.4
liver
-
Cavia porcellus
-
Storage Stability
EC Number
Storage Stability
Organism
2.8.2.4
-20°C, 25 mM monothiolglycerol, 20% loss of activity in 1 week
Cavia porcellus
2.8.2.4
-20°C, partially purified enzyme exhibits large losses of activity within 1 week
Cavia porcellus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
-
645877
Cavia porcellus
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
645877
Cavia porcellus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645877
Cavia porcellus
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645877
Cavia porcellus
r
2.8.2.4
additional information
activity toward dehydroepiandrosterone and testosterone is minimal or undetectable
645877
Cavia porcellus
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.2.4
8
-
liver isoenzyme
Cavia porcellus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.2.4
monothiolglycerol
activity is stimulated 10fold by 25 mM
Cavia porcellus
General Stability (protein specific)
EC Number
General Stability
Organism
2.8.2.4
not stabilized by 20% glycerol or 25 mM NaCl
Cavia porcellus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.4
ZnCl2
inhibition of liver isoenzyme at 15 mM
Cavia porcellus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.2.4
cytosol
-
Cavia porcellus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.8.2.4
Ca2+
activity is stimulated 2fold by 15 mM CaCl2
Cavia porcellus
2.8.2.4
Mg2+
activity is stimulated 2fold by 15 mM MgCl2
Cavia porcellus
2.8.2.4
Zn2+
weakly stimulatory at 2.5 mM ZnCl2
Cavia porcellus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.4
50000
-
chorion enzyme, gel filtration
Cavia porcellus
2.8.2.4
52300
-
liver enzyme, gel filtration
Cavia porcellus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
Cavia porcellus
-
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
Cavia porcellus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Cavia porcellus
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Cavia porcellus
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.2.4
partial, liver and chorion isoenzymes
Cavia porcellus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
chorion
-
Cavia porcellus
-
2.8.2.4
liver
-
Cavia porcellus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.8.2.4
-20°C, 25 mM monothiolglycerol, 20% loss of activity in 1 week
Cavia porcellus
2.8.2.4
-20°C, partially purified enzyme exhibits large losses of activity within 1 week
Cavia porcellus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
-
645877
Cavia porcellus
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
645877
Cavia porcellus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645877
Cavia porcellus
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645877
Cavia porcellus
r
2.8.2.4
additional information
activity toward dehydroepiandrosterone and testosterone is minimal or undetectable
645877
Cavia porcellus
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.2.4
8
-
liver isoenzyme
Cavia porcellus