BRENDA - Enzyme Database show

Enzymic synthesis of steroid sulphates. X. Isolation of oestrogen sulphotransferase from bovine placenta and comparison of its properties with adrenal oestrogen sulphotransferase

Adams, J.B.; Low, J.; Biochim. Biophys. Acta 370, 189-196 (1974)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.2.4
2-mercaptoethanol
activation
Bos taurus
2.8.2.4
dithiothreitol
activation up to 0.005 mM
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.4
dithiothreitol
inhibition above 0.005 mM
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.4
74000
76000
SDS-PAGE
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Bos taurus
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Bos taurus
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.2.4
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.2.4
placental enzyme
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
adrenal gland
-
Bos taurus
-
2.8.2.4
placenta
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.2.4
0.01
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estriol
-
645870
Bos taurus
adenosine 3',5'-bisphosphate + estriol 3-sulfate
-
645870
Bos taurus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645870
Bos taurus
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645870
Bos taurus
r
2.8.2.4
additional information
dehydroepiandrosterone, etiocholanolone, 11-deoxycorticosterone, 17beta-oestradiol-3-methyl ether, testosterone, pregnenolone, beta-naphthylamine and p-nitrophenol are no substrates
645870
Bos taurus
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.2.4
6.2
-
placental enzyme
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.8.2.4
5.5
8.5
-
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.2.4
2-mercaptoethanol
activation
Bos taurus
2.8.2.4
dithiothreitol
activation up to 0.005 mM
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.4
dithiothreitol
inhibition above 0.005 mM
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.4
74000
76000
SDS-PAGE
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Bos taurus
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Bos taurus
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.2.4
placental enzyme
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
adrenal gland
-
Bos taurus
-
2.8.2.4
placenta
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.2.4
0.01
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estriol
-
645870
Bos taurus
adenosine 3',5'-bisphosphate + estriol 3-sulfate
-
645870
Bos taurus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645870
Bos taurus
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645870
Bos taurus
r
2.8.2.4
additional information
dehydroepiandrosterone, etiocholanolone, 11-deoxycorticosterone, 17beta-oestradiol-3-methyl ether, testosterone, pregnenolone, beta-naphthylamine and p-nitrophenol are no substrates
645870
Bos taurus
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.2.4
6.2
-
placental enzyme
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.8.2.4
5.5
8.5
-
Bos taurus