BRENDA - Enzyme Database show

Enzymic synthesis of steroid sulphates. IX. Physical and chemical properties of purified oestrogen sulphotransferase from bovine adrenal glands, the nature of its isoenzymic forms and a proposed model to explain its wave-like kinetics

Adams, J.B.; Ellyard, R.K.; Low, J.; Biochim. Biophys. Acta 370, 160-188 (1974)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.2.4
2-mercaptoethanol
activation
Bos taurus
2.8.2.4
cysteine
-
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.4
3'-AMP
-
Bos taurus
2.8.2.4
3'-phosphoadenylylsulfate
competitive inhibition
Bos taurus
2.8.2.4
ADP
non-competitive inhibitor
Bos taurus
2.8.2.4
ATP
mixed-type inhibition
Bos taurus
2.8.2.4
p-hydroxymercuribenzoate
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.2.4
0.015
-
estrone
pH 8.1, 37°C
Bos taurus
2.8.2.4
0.037
-
3'-phosphoadenylylsulfate
pH 8.1, 37°C
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.4
73800
-
sedimentation equilibrium centrifugation
Bos taurus
2.8.2.4
74000
-
sucrose density gradient, pH 5.8
Bos taurus
2.8.2.4
74800
-
meniscus depletion method, sucrose density gradient centrifugation, pH 7.0
Bos taurus
2.8.2.4
76000
-
sucrose density gradient centrifugation, pH 9.0
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Bos taurus
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Bos taurus
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.2.4
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.2.4
adrenal enzyme
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
adrenal gland
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + 17beta-estradiol
-
645869
Bos taurus
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
645869
Bos taurus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645869
Bos taurus
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645869
Bos taurus
r
Subunits
EC Number
Subunits
Commentary
Organism
2.8.2.4
monomer
1 * 74000, SDS-PAGE
Bos taurus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.8.2.4
0.056
-
3'-phosphoadenylylsulfate
pH 8.1, 37°C
Bos taurus
2.8.2.4
1.9
-
3'-AMP
pH 8.1, 37°C
Bos taurus
2.8.2.4
2.8
-
ADP
pH 8.1, 37°C
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.2.4
2-mercaptoethanol
activation
Bos taurus
2.8.2.4
cysteine
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.4
3'-AMP
-
Bos taurus
2.8.2.4
3'-phosphoadenylylsulfate
competitive inhibition
Bos taurus
2.8.2.4
ADP
non-competitive inhibitor
Bos taurus
2.8.2.4
ATP
mixed-type inhibition
Bos taurus
2.8.2.4
p-hydroxymercuribenzoate
-
Bos taurus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.8.2.4
0.056
-
3'-phosphoadenylylsulfate
pH 8.1, 37°C
Bos taurus
2.8.2.4
1.9
-
3'-AMP
pH 8.1, 37°C
Bos taurus
2.8.2.4
2.8
-
ADP
pH 8.1, 37°C
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.2.4
0.015
-
estrone
pH 8.1, 37°C
Bos taurus
2.8.2.4
0.037
-
3'-phosphoadenylylsulfate
pH 8.1, 37°C
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.4
73800
-
sedimentation equilibrium centrifugation
Bos taurus
2.8.2.4
74000
-
sucrose density gradient, pH 5.8
Bos taurus
2.8.2.4
74800
-
meniscus depletion method, sucrose density gradient centrifugation, pH 7.0
Bos taurus
2.8.2.4
76000
-
sucrose density gradient centrifugation, pH 9.0
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Bos taurus
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Bos taurus
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.2.4
adrenal enzyme
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
adrenal gland
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + 17beta-estradiol
-
645869
Bos taurus
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
645869
Bos taurus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645869
Bos taurus
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645869
Bos taurus
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.8.2.4
monomer
1 * 74000, SDS-PAGE
Bos taurus