BRENDA - Enzyme Database show

Enzymic synthesis of steroid sulphates. III. Isolation and properties of estrogen sulphotransferase of bovine adrenal glands

Adams, J.B.; Poulos, A.; Biochim. Biophys. Acta 146, 493-508 (1967)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.2.4
cysteine
-
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.4
ADP
non-competitive inhibition
Bos taurus
2.8.2.4
Co2+
strong inhibition
Bos taurus
2.8.2.4
additional information
only moderately sensitive to SH-blocking agents, no inhibition with EDTA up to 20 mM
Bos taurus
2.8.2.4
Ni2+
strong inhibition
Bos taurus
2.8.2.4
o-Iodosobenzoate
sensitive above 10 mM
Bos taurus
2.8.2.4
p-chloromercuribenzoate
sensitive above 1 mM
Bos taurus
2.8.2.4
Zn2+
strong inhibition
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.2.4
0.014
-
17beta-estradiol
pH 8.1, 37°C
Bos taurus
2.8.2.4
0.07
-
adenosine 3',5'-bisphosphate
pH 8.1, 37°C
Bos taurus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.8.2.4
Ca2+
activated by, but no absolute requirement
Bos taurus
2.8.2.4
Mg2+
activated by, but no absolute requirement
Bos taurus
2.8.2.4
Mn2+
activated by, but no absolute requirement
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Bos taurus
steroid metabolism
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Bos taurus
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.2.4
Bos taurus
-
bovine
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.2.4
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
adrenal gland
-
Bos taurus
-
Storage Stability
EC Number
Storage Stability
Organism
2.8.2.4
0°C, partially purified A form of enzyme loses only a small percentage of its activity on standing in ice for a period of 2 weeks, B form loses 50% of its initial activity in this time
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + 17beta-estradiol
-
645867
Bos taurus
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
645867
Bos taurus
r
2.8.2.4
3'-phosphoadenylylsulfate + estriol
-
645867
Bos taurus
adenosine 3',5'-bisphosphate + estriol 3-sulfate
-
645867
Bos taurus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645867
Bos taurus
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645867
Bos taurus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
steroid metabolism
645867
Bos taurus
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645867
Bos taurus
r
2.8.2.4
3'-phosphoadenylylsulfate + hexestrol
-
645867
Bos taurus
adenosine 3',5'-bisphosphate + hexestrol 3-sulfate
-
645867
Bos taurus
r
2.8.2.4
3'-phosphoadenylylsulfate + stilbestrol
-
645867
Bos taurus
adenosine 3',5'-bisphosphate + stilbestrol 4-sulfate
-
645867
Bos taurus
r
2.8.2.4
additional information
simple phenols, 2-naphthylamine and 3beta-hydroxysteroids are not sulfated, dienestrol is no substrate, dehydroepiandrosterone, phenol, p-nitrophenol, alpha-naphthol, beta-naphthol, beta-naphthylamine and 17 beta-estradiol-3-methyl ether fail to act as substrates
645867
Bos taurus
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.2.4
8
-
-
Bos taurus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.8.2.4
1.1
-
ADP
pH 8.1, 37°C
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.2.4
cysteine
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.4
ADP
non-competitive inhibition
Bos taurus
2.8.2.4
Co2+
strong inhibition
Bos taurus
2.8.2.4
additional information
only moderately sensitive to SH-blocking agents, no inhibition with EDTA up to 20 mM
Bos taurus
2.8.2.4
Ni2+
strong inhibition
Bos taurus
2.8.2.4
o-Iodosobenzoate
sensitive above 10 mM
Bos taurus
2.8.2.4
p-chloromercuribenzoate
sensitive above 1 mM
Bos taurus
2.8.2.4
Zn2+
strong inhibition
Bos taurus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.8.2.4
1.1
-
ADP
pH 8.1, 37°C
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.2.4
0.014
-
17beta-estradiol
pH 8.1, 37°C
Bos taurus
2.8.2.4
0.07
-
adenosine 3',5'-bisphosphate
pH 8.1, 37°C
Bos taurus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.8.2.4
Ca2+
activated by, but no absolute requirement
Bos taurus
2.8.2.4
Mg2+
activated by, but no absolute requirement
Bos taurus
2.8.2.4
Mn2+
activated by, but no absolute requirement
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Bos taurus
steroid metabolism
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Bos taurus
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.2.4
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
adrenal gland
-
Bos taurus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.8.2.4
0°C, partially purified A form of enzyme loses only a small percentage of its activity on standing in ice for a period of 2 weeks, B form loses 50% of its initial activity in this time
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + 17beta-estradiol
-
645867
Bos taurus
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
645867
Bos taurus
r
2.8.2.4
3'-phosphoadenylylsulfate + estriol
-
645867
Bos taurus
adenosine 3',5'-bisphosphate + estriol 3-sulfate
-
645867
Bos taurus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645867
Bos taurus
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645867
Bos taurus
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
steroid metabolism
645867
Bos taurus
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645867
Bos taurus
r
2.8.2.4
3'-phosphoadenylylsulfate + hexestrol
-
645867
Bos taurus
adenosine 3',5'-bisphosphate + hexestrol 3-sulfate
-
645867
Bos taurus
r
2.8.2.4
3'-phosphoadenylylsulfate + stilbestrol
-
645867
Bos taurus
adenosine 3',5'-bisphosphate + stilbestrol 4-sulfate
-
645867
Bos taurus
r
2.8.2.4
additional information
simple phenols, 2-naphthylamine and 3beta-hydroxysteroids are not sulfated, dienestrol is no substrate, dehydroepiandrosterone, phenol, p-nitrophenol, alpha-naphthol, beta-naphthol, beta-naphthylamine and 17 beta-estradiol-3-methyl ether fail to act as substrates
645867
Bos taurus
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.2.4
8
-
-
Bos taurus