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Literature summary extracted from

  • Kwon, A.R.; Yun, H.J.; Choi, E.C.
    Kinetic mechanism and identification of the active site tyrosine residue in Enterobacter amnigenus arylsulfate sulfotransferase (2001), Biochem. Biophys. Res. Commun., 285, 526-529.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.22
-
 Lelliottia amnigena

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.22 Y101F reduced enzyme activity Lelliottia amnigena
2.8.2.22 Y123F severely reduced enzyme activity Lelliottia amnigena
2.8.2.22 Y160F reduced enzyme activity Lelliottia amnigena
2.8.2.22 Y389F reduced enzyme activity Lelliottia amnigena
2.8.2.22 Y47F reduced enzyme activity Lelliottia amnigena
2.8.2.22 Y494F enzyme activity similar to wild-type enzyme Lelliottia amnigena
2.8.2.22 Y500F reduced enzyme activity Lelliottia amnigena
2.8.2.22 Y515F reduced enzyme activity Lelliottia amnigena
2.8.2.22 Y533F reduced enzyme activity Lelliottia amnigena
2.8.2.22 Y82F reduced enzyme activity Lelliottia amnigena
2.8.2.22 Y92F increased enzyme activity Lelliottia amnigena

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.22 1-naphthyl sulfate competitive with 4-nitrophenyl sulfate, noncompetitive with 1-naphthol Lelliottia amnigena
2.8.2.22 4-nitrophenol noncompetitive with respect to 4-nitrophenyl sulfate, competitive with respect to alpha-naphthol Lelliottia amnigena

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.22 Lelliottia amnigena Q9XDP2 AR-37
-
2.8.2.22 Lelliottia amnigena AR-37 Q9XDP2 AR-37
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.22
-
 Lelliottia amnigena

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.2.22 an aryl sulfate + a phenol = a phenol + an aryl sulfate mechanism Lelliottia amnigena
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.22 4-nitrophenyl sulfate + alpha-naphthol
-
 Lelliottia amnigena 4-nitrophenol + 1-naphththyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + alpha-naphthol
-
 Lelliottia amnigena AR-37 4-nitrophenol + 1-naphththyl sulfate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.22 0.15
-
 4-nitrophenol pH 9, 37°C, for alpha-naphthol Lelliottia amnigena
2.8.2.22 0.87
-
 4-nitrophenol pH 9, 37°C, for 4-nitrophenyl sulfate Lelliottia amnigena
2.8.2.22 1.63
-
 1-naphthyl sulfate pH 9, 37°C, for 1-naphthol Lelliottia amnigena
2.8.2.22 2.68
-
 1-naphthyl sulfate pH 9, 37°C, for 4-nitrophenyl sulfate Lelliottia amnigena