BRENDA - Enzyme Database show

Purification and characterization of novel sulfotransferase obtained from Klebsiella K-36, an intestinal bacterium of rat

Kim, D.H.; Kim, H.S.; Kobashi, K.; J. Biochem. 112, 456-460 (1992)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.22
Ca2+
33% inhibition at 2 mM
Klebsiella sp.
2.8.2.22
Cd2+
almost complete inhibition at 2 mM
Klebsiella sp.
2.8.2.22
Cu2+
37% inhibition at 2 mM
Klebsiella sp.
2.8.2.22
EDTA
inactivation reversed by addition of metal ions
Klebsiella sp.
2.8.2.22
Ni2+
32% inhibition at 2 mM
Klebsiella sp.
2.8.2.22
Pb2+
35% inhibition at 2 mM
Klebsiella sp.
2.8.2.22
Zn2+
38% inhibition at 2 mM
Klebsiella sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.2.22
0.11
-
4-nitrophenyl sulfate
with phenol as second substrate, pH 8, 37°C
Klebsiella sp.
2.8.2.22
0.66
-
phenol
with 4-nitrophenyl sulfate as second substrate, pH 8, 37°C
Klebsiella sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.8.2.22
Co2+
33% increase at 2 mM
Klebsiella sp.
2.8.2.22
Mg2+
65% increase at 2 mM
Klebsiella sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.22
73000
-
2 * 73000, SDS-PAGE
Klebsiella sp.
2.8.2.22
160000
-
gel filtration
Klebsiella sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.22
additional information
Klebsiella sp.
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
?
-
-
-
2.8.2.22
additional information
Klebsiella sp. K-36
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.2.22
Klebsiella sp.
-
K-36
-
2.8.2.22
Klebsiella sp. K-36
-
K-36
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.2.22
-
Klebsiella sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.2.22
8.82
-
-
Klebsiella sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.22
4-acetylphenyl sulfate + phenol
28% of the activity with 4-nitrophenyl sulfate
645840
Klebsiella sp.
4-acetylphenol + phenyl sulfate
-
-
-
?
2.8.2.22
4-methylumbelliferyl sulfate + phenol
55% of the activity with p-nitrophenyl sulfate
645840
Klebsiella sp.
4-methylumbelliferol + phenyl sulfate
-
-
-
?
2.8.2.22
4-nitrophenyl sulfate + 3-chlorophenol
-
645840
Klebsiella sp.
3-chlorophenyl sulfate + 4-nitrophenol
-
-
-
?
2.8.2.22
4-nitrophenyl sulfate + 3-chlorophenol
-
645840
Klebsiella sp. K-36
3-chlorophenyl sulfate + 4-nitrophenol
-
-
-
?
2.8.2.22
4-nitrophenyl sulfate + 9-phenanthrol
-
645840
Klebsiella sp.
9-phenanthroyl sulfate + 4-nitrophenol
-
-
-
?
2.8.2.22
4-nitrophenyl sulfate + 9-phenanthrol
-
645840
Klebsiella sp. K-36
9-phenanthroyl sulfate + 4-nitrophenol
-
-
-
?
2.8.2.22
4-nitrophenyl sulfate + alpha-naphthol
-
645840
Klebsiella sp.
4-nitrophenol + 1-napththyl sulfate
-
-
-
?
2.8.2.22
4-nitrophenyl sulfate + alpha-naphthol
-
645840
Klebsiella sp. K-36
4-nitrophenol + 1-napththyl sulfate
-
-
-
?
2.8.2.22
4-nitrophenyl sulfate + phenol
-
645840
Klebsiella sp.
phenyl sulfate + 4-nitrophenol
-
-
-
?
2.8.2.22
estrone sulfate + phenol
11% of the activity with p-nitrophenyl sulfate
645840
Klebsiella sp.
estrone + phenyl sulfate
-
-
-
?
2.8.2.22
additional information
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
645840
Klebsiella sp.
?
-
-
-
-
2.8.2.22
additional information
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
645840
Klebsiella sp. K-36
?
-
-
-
-
2.8.2.22
phenyl sulfate + an aryl alcohol
-
645840
Klebsiella sp.
phenol + an aryl sulfate
-
-
-
?
2.8.2.22
phenyl sulfate + an aryl alcohol
-
645840
Klebsiella sp. K-36
phenol + an aryl sulfate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.8.2.22
dimer
2 * 73000, SDS-PAGE
Klebsiella sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.8.2.22
37
-
assay at
Klebsiella sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.8.2.22
50
-
10 min, stable below
Klebsiella sp.
2.8.2.22
60
-
10 min, inactivation
Klebsiella sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.2.22
10
10.5
-
Klebsiella sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.22
Ca2+
33% inhibition at 2 mM
Klebsiella sp.
2.8.2.22
Cd2+
almost complete inhibition at 2 mM
Klebsiella sp.
2.8.2.22
Cu2+
37% inhibition at 2 mM
Klebsiella sp.
2.8.2.22
EDTA
inactivation reversed by addition of metal ions
Klebsiella sp.
2.8.2.22
Ni2+
32% inhibition at 2 mM
Klebsiella sp.
2.8.2.22
Pb2+
35% inhibition at 2 mM
Klebsiella sp.
2.8.2.22
Zn2+
38% inhibition at 2 mM
Klebsiella sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.2.22
0.11
-
4-nitrophenyl sulfate
with phenol as second substrate, pH 8, 37°C
Klebsiella sp.
2.8.2.22
0.66
-
phenol
with 4-nitrophenyl sulfate as second substrate, pH 8, 37°C
Klebsiella sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.8.2.22
Co2+
33% increase at 2 mM
Klebsiella sp.
2.8.2.22
Mg2+
65% increase at 2 mM
Klebsiella sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.22
73000
-
2 * 73000, SDS-PAGE
Klebsiella sp.
2.8.2.22
160000
-
gel filtration
Klebsiella sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.22
additional information
Klebsiella sp.
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
?
-
-
-
2.8.2.22
additional information
Klebsiella sp. K-36
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.2.22
-
Klebsiella sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.2.22
8.82
-
-
Klebsiella sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.22
4-acetylphenyl sulfate + phenol
28% of the activity with 4-nitrophenyl sulfate
645840
Klebsiella sp.
4-acetylphenol + phenyl sulfate
-
-
-
?
2.8.2.22
4-methylumbelliferyl sulfate + phenol
55% of the activity with p-nitrophenyl sulfate
645840
Klebsiella sp.
4-methylumbelliferol + phenyl sulfate
-
-
-
?
2.8.2.22
4-nitrophenyl sulfate + 3-chlorophenol
-
645840
Klebsiella sp.
3-chlorophenyl sulfate + 4-nitrophenol
-
-
-
?
2.8.2.22
4-nitrophenyl sulfate + 3-chlorophenol
-
645840
Klebsiella sp. K-36
3-chlorophenyl sulfate + 4-nitrophenol
-
-
-
?
2.8.2.22
4-nitrophenyl sulfate + 9-phenanthrol
-
645840
Klebsiella sp.
9-phenanthroyl sulfate + 4-nitrophenol
-
-
-
?
2.8.2.22
4-nitrophenyl sulfate + 9-phenanthrol
-
645840
Klebsiella sp. K-36
9-phenanthroyl sulfate + 4-nitrophenol
-
-
-
?
2.8.2.22
4-nitrophenyl sulfate + alpha-naphthol
-
645840
Klebsiella sp.
4-nitrophenol + 1-napththyl sulfate
-
-
-
?
2.8.2.22
4-nitrophenyl sulfate + alpha-naphthol
-
645840
Klebsiella sp. K-36
4-nitrophenol + 1-napththyl sulfate
-
-
-
?
2.8.2.22
4-nitrophenyl sulfate + phenol
-
645840
Klebsiella sp.
phenyl sulfate + 4-nitrophenol
-
-
-
?
2.8.2.22
estrone sulfate + phenol
11% of the activity with p-nitrophenyl sulfate
645840
Klebsiella sp.
estrone + phenyl sulfate
-
-
-
?
2.8.2.22
additional information
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
645840
Klebsiella sp.
?
-
-
-
-
2.8.2.22
additional information
may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation
645840
Klebsiella sp. K-36
?
-
-
-
-
2.8.2.22
phenyl sulfate + an aryl alcohol
-
645840
Klebsiella sp.
phenol + an aryl sulfate
-
-
-
?
2.8.2.22
phenyl sulfate + an aryl alcohol
-
645840
Klebsiella sp. K-36
phenol + an aryl sulfate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.8.2.22
dimer
2 * 73000, SDS-PAGE
Klebsiella sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.8.2.22
37
-
assay at
Klebsiella sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.8.2.22
50
-
10 min, stable below
Klebsiella sp.
2.8.2.22
60
-
10 min, inactivation
Klebsiella sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.2.22
10
10.5
-
Klebsiella sp.