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Literature summary extracted from

  • Yamamoto, Y.; Takahashi, I.; Ogata, N.; Nakazawa, K.
    Purification and characterization of N-acetylglucosaminyl sulfotransferase from chick corneas (2001), Arch. Biochem. Biophys., 392, 87-92.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.21 protamine 2fold activation at 0.01% Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.21 N-acetylgalactosamine slight inhibition at 25 mM Gallus gallus
2.8.2.21 N-acetylglucosamine 39% inhibition at 25 mM Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.21 Mn2+ 26% stimulation at 5 mM Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.21 55000
-
 SDS-PAGE, 2 bands with 55000 Da and 66000 Da Gallus gallus
2.8.2.21 66000
-
 native PAGE Gallus gallus
2.8.2.21 66000
-
 SDS-PAGE, 2 bands with 55000 Da and 66000 Da Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.21 additional information Gallus gallus sulfation of GlcNAc is coupled to enlongation of keratan sulfate chain ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.21 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.21
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.21 cornea
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.21 0.000033
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.21 3'-phosphoadenylylsulfate + pyridylamino-GlcNAcbeta1-3Galbeta1-4GlcNAc sulfation at GlcNAc residues at the nonreducing end but no sulfation of internal GlcNAc Gallus gallus adenosine 3',5'-bisphosphate + pyridylamino-GlcNAcbeta1-3Galbeta1-4(6-sulfo)GlcNAc
-
 ?
2.8.2.21 additional information N-acetylglucosamine residues do not act as acceptors Gallus gallus ?
-
 ?
2.8.2.21 additional information sulfation of GlcNAc is coupled to enlongation of keratan sulfate chain Gallus gallus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.21 37
-
 assay at Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.21 6
-
 MES buffer Gallus gallus