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Literature summary extracted from

  • Keller, R.; Driesch, R.; Stein, T.; Momburg, M.; Stuhlsatz, H.W.; Greiling, H.
    Biosynthesis of proteokeratan sulfate in the bovine cornea. 1. Isolation and characterization of a keratan sulfotransferase and the role of sulfation for the chain termination (1983), Hoppe-Seyler's Z. Physiol. Chem., 364, 239-252.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.21 2'-AMP weak, above 0.08 mM Bos taurus
2.8.2.21 3'-AMP weak, above 0.08 mM Bos taurus
2.8.2.21 5'-adenylylsulfate
-
 Bos taurus
2.8.2.21 5'-AMP weak, above 0.08 mM Bos taurus
2.8.2.21 ADP above 0.08 mM Bos taurus
2.8.2.21 AMP above 0.08 mM Bos taurus
2.8.2.21 ATP above 0.08 mM Bos taurus
2.8.2.21 cAMP weak Bos taurus
2.8.2.21 Cu2+ above 1 mM, complete inhibition above 2 mM Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.21 0.025
-
 Keratan sulfate pH 6, 15°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.21 cytosol
-
 Bos taurus 5829
-
2.8.2.21 microsome
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.21 Ca2+ activates, optimal concentration: 5 mM Bos taurus
2.8.2.21 Co2+ activates, optimal concentration: 5 mM Bos taurus
2.8.2.21 Mn2+ activates, optimal concentration: 5 mM Bos taurus
2.8.2.21 Zn2+ activates, optimal concentration: 5 mM Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.21 240000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.21 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.21 244fold Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.21 cornea
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.21 0.000013
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.21 3'-phosphoadenylylsulfate + keratan sulfate activity decreases with increasing molecular mass and sulfation degree of keratan sulfate Bos taurus adenosine 3',5'-bisphosphate + keratan 6'-sulfate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.21 12
-
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.21 6
-
 2 optima: pH 6.0 and 8.6 Bos taurus
2.8.2.21 8.6
-
 2 optima: pH 6.0 and 8.6 Bos taurus