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Literature summary extracted from

  • Niehrs, C.; Kraft, M.; Lee, R.W.H.; Huttner, W.B.
    Analysis of the substrate specificity of tyrosylprotein sulfotransferase using synthetic peptides (1990), J. Biol. Chem., 265, 8525-8532.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.20 additional information
-
 additional information kinetic properties with synthetic peptides as substrates Bos taurus
2.8.2.20 additional information
-
 additional information the Km-values of peptides with multiple Tyr-sulfation sites decrease exponentially with the number of sites Bos taurus
2.8.2.20 0.000044
-
 KKE(EPEYGE)3 pH 6.5, 30°C Bos taurus
2.8.2.20 0.00034
-
 KKEEPEYGE(EPEFGE)2 pH 6.5, 30°C Bos taurus
2.8.2.20 0.0017
-
 KKE(EPEYGE)2 pH 6.5, 30°C Bos taurus
2.8.2.20 0.0019 0.002 3'-phosphoadenylylsulfate + peptide CCKI Bos taurus
2.8.2.20 0.035
-
 cholecystokinin I pH 6.5, 30°C Bos taurus
2.8.2.20 0.043
-
 KKEEPEYGE pH 6.5, 30°C Bos taurus
2.8.2.20 0.12
-
 cholecystokinin-3 pH 6.5, 30°C Bos taurus
2.8.2.20 0.14
-
 peptide tub-1 pH 6.5, 30°C Bos taurus
2.8.2.20 0.15
-
 cholecystokinin-2 pH 6.5, 30°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.20 Golgi membrane
-
 Bos taurus 139
-
2.8.2.20 membrane
-
 Bos taurus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.20 Mn2+ activation Bos taurus
2.8.2.20 Mn2+ 5 mM Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.20 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.20 adrenal medulla
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.20 3'-phosphoadenylyl sulfate + cholecystokinin I
-
 Bos taurus ?
-
 ?
2.8.2.20 3'-phosphoadenylyl sulfate + cholecystokinin-2
-
 Bos taurus ?
-
 ?
2.8.2.20 3'-phosphoadenylyl sulfate + cholecystokinin-3
-
 Bos taurus ?
-
 ?
2.8.2.20 3'-phosphoadenylyl sulfate + peptide tub-1
-
 Bos taurus ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + acidic polypeptide tyrosine specifically sulfates Tyr-residues adjacent to acidic amino acids Bos taurus adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + KKE(EPEYGE)2
-
 Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + KKE(EPEYGE)3
-
 Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + KKEEPEYGE
-
 Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + KKEEPEYGE(EPEFGE)2
-
 Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + synthetic peptides peptides modelled after known or putative Tyr-sulfation sites of chromogranin B, i.e. cholecystokinin precursor, peptides CCK-1 and variants, secretogranin I, peptides SgI-1 to SgI-4, vitronectin modeled after phosphorylation site of alpha-tubulin or autophosphorylation site of pp60src, pp60v-src(Tyr-416) Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 additional information structural determinants for substrate specificity Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.20 30
-
 assay at Bos taurus