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Literature summary extracted from

  • Niehrs, C.; Huttner, W.B.
    Purification and characterization of tyrosylprotein sulfotransferase (1990), EMBO J., 9, 35-42.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.20 additional information
-
 additional information kinetic properties with synthetic peptides as substrates Bos taurus
2.8.2.20 0.0014
-
 3'-phosphoadenylylsulfate
-
 Bos taurus
2.8.2.20 0.021
-
 KKSAEDYEYPSMG pH 6.5, 30°C Bos taurus
2.8.2.20 0.37
-
 KKSAEDYQYPSMG pH 6.5, 30°C Bos taurus
2.8.2.20 0.55
-
 KKSAENYEYPSMG pH 6.5, 30°C Bos taurus
2.8.2.20 0.59
-
 KKSAENYQYPSMG pH 6.5, 30°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.20 Golgi membrane trans-most subcompartment Bos taurus 139
-
2.8.2.20 membrane integral membrane-protein Bos taurus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.20 Mg2+ requirement Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.20 100000
-
 enzyme-detergent micelle, glycerol gradient centrifugation Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.20 3'-phosphoadenylylsulfate + protein tyrosine Bos taurus post-translational modification of biologically active peptides and proteins adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.20 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.8.2.20 glycoprotein
-
 Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.20 treatment of membrane-bound enzyme with carbonate, followed by solubilization and affinity chromatography on a substrate peptide Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.20 adrenal medulla
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.20 0.0057
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.8.2.20 4°C, detergent-solubilized enzyme preparation, t1/2: 48 h Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.20 3'-phosphoadenylylsulfate + acidic polypeptide tyrosine specifically sulfates Tyr-residues adjacent to acidic amino acids Bos taurus adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + alpha-tubulin peptide PKV Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + KKSAEDYEYPSMG
-
 Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + KKSAEDYQYPSMG
-
 Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + KKSAENYEYPSMG
-
 Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + KKSAENYQYPSMG
-
 Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + PKG
-
 Bos taurus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + protein tyrosine post-translational modification of biologically active peptides and proteins Bos taurus adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.20 ? x * 50000-54000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.20 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.20 6
-
 PKG Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.20 5.7 6.8 about half-maximal activity at pH 5.7 and 6.8 Bos taurus