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Literature summary extracted from

  • Rearick, J.I.; Calhoun, E.S.
    Purification and characterization of cholesterol sulfotransferase from rat skin (2001), Biochem. Cell Biol., 79, 499-506.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.2 additional information stimulated by nonionic detergents Rattus norvegicus

General Stability

EC Number General Stability Organism
2.8.2.2 partially purified enzyme loses 30% of upon freeze-thawing, activity can be stabilized by adenosine 3',5'-bisphosphate Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.2 adenosine 3',5'-bisphosphate competitive inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.2 0.00063
-
 3beta,20beta-pregnenediol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.0016
-
 pregnenolone pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.0025
-
 cholesterol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.0086
-
 dehydroepiandrosterone pH 7.5, 37°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.2 40000
-
 SDS-PAGE Rattus norvegicus
2.8.2.2 50000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.2 3'-phosphoadenylylsulfate + dehydroepiandrosterone Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.2 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.2 epidermis epidermal keratinocyte Rattus norvegicus
-
2.8.2.2 epithelium
-
 Rattus norvegicus
-
2.8.2.2 skin
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.2 0.0084
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.8.2.2 -20°C, partially purified enzyme in 50% glycerol or ethylene glycol loses 30% of activity overnight Rattus norvegicus
2.8.2.2 4°C, partially purified enzyme loses 30% of activity overnight Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.2 3'-phosphoadenylyl sulfate + 3beta,20beta-pregnenediol
-
 Rattus norvegicus ?
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + cholesterol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + cholesterol sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + pregn-5-ene-3beta,20beta-diol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + (3alpha,20S)-20-hydroxypregn-5-en-3-yl sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + pregnenolone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 5-pregnen-20-one 3-sulfate
-
 r
2.8.2.2 additional information 3beta-hydroxysteroids with a DELTA5 double bond serve as substrates while androgens, estrogens, corticosteroids, p-nitrophenol and 3,4-dihydroxyphenylalanine do not serve as substrates Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.2 8
-
 broad pH optimum centered on pH 8.0 Rattus norvegicus