Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lyon, E.S.; Marcus, C.J.; Wang, J.L.; Jakoby, W.B.
    Hydroxysteroid sulfotransferase (1981), Methods Enzymol., 77, 206-213.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.2 0.012
-
3'-phosphoadenylylsulfate pH 5.5, 37°C Rattus norvegicus
2.8.2.2 0.012
-
dehydroepiandrosterone pH 5.5, 37°C Rattus norvegicus
2.8.2.2 0.035
-
beta-estradiol pH 5.5, 37°C Rattus norvegicus
2.8.2.2 0.07
-
testosterone pH 5.5, 37°C Rattus norvegicus
2.8.2.2 0.29
-
beta-estradiol pH 5.5, 37°C Rattus norvegicus
2.8.2.2 1.2
-
Isoamylalcohol pH 5.5, 37°C Rattus norvegicus
2.8.2.2 1.7
-
1-amylalcohol pH 5.5, 37°C Rattus norvegicus
2.8.2.2 3
-
1-butanol pH 5.5, 37°C Rattus norvegicus
2.8.2.2 42
-
ethanol pH 5.5, 37°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.2 28000
-
6 * 28000, SDS-PAGE Rattus norvegicus
2.8.2.2 180000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.2 3'-phosphoadenylylsulfate + dehydroepiandrosterone Rattus norvegicus
-
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.2 Rattus norvegicus
-
rat
-
2.8.2.2 Rattus norvegicus
-
Sprague-Dawley, female
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.2 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.2 0.57
-
-
Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.8.2.2 -80°C, stable for several months Rattus norvegicus
2.8.2.2 4°C, loses 10% activity per week upon storage Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.2 3'-phosphoadenylylsulfate + 1-butanol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 1-butanoyl sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + 11-deoxycorticosterone
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + 2-propanol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 2-propyl sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + aldosterone
-
Rattus norvegicus adenosine 3',5'-bisphosphate + aldosterone sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + amyl alcohol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + amyl sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + androst-5-en-3beta,17alpha-diol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
r
2.8.2.2 3'-phosphoadenylylsulfate + ascorbic acid
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
r
2.8.2.2 3'-phosphoadenylylsulfate + beta-estradiol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + beta-estradiol 3-O-sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + chloramphenicol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
r
2.8.2.2 3'-phosphoadenylylsulfate + corticosterone
-
Rattus norvegicus adenosine 3',5'-bisphosphate + corticosterone sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + cortisol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + cortisol sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + D-propranolol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
r
2.8.2.2 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
Rattus norvegicus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + dehydroepiandrosterone 3beta-hydroxyandrost-5-en-17-one Rattus norvegicus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + ephedrine
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ephedrine sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + ethanol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ethyl sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + hydrocortisone
-
Rattus norvegicus adenosine 3',5'-bisphosphate + hydrocortisone sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + isoamyl alcohol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + isoamyl sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + L-propranolol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
r
2.8.2.2 3'-phosphoadenylylsulfate + ouabain
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
r
2.8.2.2 3'-phosphoadenylylsulfate + retinol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + retinol sulfate
-
r
2.8.2.2 3'-phosphoadenylylsulfate + testosterone 17beta-hydroxyandrost-4-en-3-one Rattus norvegicus adenosine 3',5'-bisphosphate + testosterone 3-sulfate
-
r
2.8.2.2 additional information does not utilize cholesterol, 2-naphthylamine, 2-naphthol, taurolithocholic acid, estrone, progesterone and N-hydroxy-2-acetylaminofluorene as sulfate acceptors Rattus norvegicus ?
-
?

Subunits

EC Number Subunits Comment Organism
2.8.2.2 hexamer 6 * 28000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.2 6
-
dehydroepiandrosterone as substrate Rattus norvegicus
2.8.2.2 7.5
-
1-butanol as substrate Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.2.2 Rattus norvegicus isoelectric focusing, sucrose gradient in presence of 2.5% ampholines pH 5.0-7.0
-
5