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Literature summary extracted from

  • Ryan, R.A.; Carroll, J.
    Studies on a 3beta-hydroxysteroid sulphotransferase from rat liver (1976), Biochim. Biophys. Acta, 429, 391-401.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.2 glutathione activity of the purified enzyme is slightly enhanced Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.2 adenosine 3',5'-bisphosphate enzyme appears to be highly suspectible to product inhibition Rattus norvegicus
2.8.2.2 HgCl2
-
 Rattus norvegicus
2.8.2.2 additional information EDTA has no effect Rattus norvegicus
2.8.2.2 p-chloromercuribenzoate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.2 0.006
-
 dehydroepiandrosterone pH 8.0, 37°C Rattus norvegicus
2.8.2.2 0.013
-
 3'-phosphoadenylylsulfate pH 8.0, 37°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.2 additional information crude enzyme activity is markedly stimulated by divalent cations like Fe2+, Co2+ and Mn2+, this effect is not observed with purified enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.2 3'-phosphoadenylylsulfate + dehydroepiandrosterone Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.2 Rattus norvegicus
-
 rat
-
2.8.2.2 Rattus norvegicus
-
 Wistar, female
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.2 partially Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.2 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.2 0.01716
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.8.2.2 -20°C, activity towards dehydroepiandrosterone as acceptor substrate is stable for several months Rattus norvegicus
2.8.2.2 4°C, activity declines by 60% after 4 weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.2 3'-phosphoadenylylsulfate + 1-butanol relative activity 7% Rattus norvegicus adenosine 3',5'-bisphosphate + 1-butanoyl sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + 11-deoxycorticosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + 11-deoxycorticosterone relative activity 10% Rattus norvegicus adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + 3alpha-hydroxy-5beta-androstan-17-one relative activity 5% Rattus norvegicus adenosine 3',5'-bisphosphate + 5beta-androstan-17-one 3-sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 3beta,17alpha-dihydroxy-pregn-5-ene-20-one relative activity 4% Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + 3beta-hydroxy-5beta-androstan-17-one relative activity 45% Rattus norvegicus adenosine 3',5'-bisphosphate + 5beta-androstan-17-one 3-sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + 5alpha-androstane-3alpha,17beta-diol relative activity 20% Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + 5alpha-androstane-3beta,17beta-diol relative activity 83% Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + 5alpha-pregnane-3beta,20alpha-diol relative activity 86% Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + 5beta-androstane-3alpha,17beta-diol relative activity 7% Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + alpha-naphthol relative activity 4% Rattus norvegicus adenosine 3',5'-bisphosphate + alpha-naphthyl sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + androst-5-en-3beta,17beta-diol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + androst-5-en-3beta,17beta-diol sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + androsterone 3alpha-hydroxy-5alpha-androstan-17-one Rattus norvegicus adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + androsterone relative activity 53% Rattus norvegicus adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + corticosterone relative activity 6% Rattus norvegicus adenosine 3',5'-bisphosphate + corticosterone sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + epiandrosterone 3beta-hydroxy-5alpha-androstan-17-one Rattus norvegicus adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + L-tyrosinemethyl ester relative activity 9% Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + pregnenolone relative activity 61% Rattus norvegicus adenosine 3',5'-bisphosphate + 5-pregnen-20-one 3-sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + pregnenolone 3beta-hydroxy-5-pregnen-20-one Rattus norvegicus adenosine 3',5'-bisphosphate + 5-pregnen-20-one 3-sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + testosterone relative activity 2% Rattus norvegicus adenosine 3',5'-bisphosphate + testosterone 3-sulfate
-
 r
2.8.2.2 additional information inactive towards estrogens, cholesterol and ergocalciferol, maximum activity is observed when the steroid is of the allo-series and possesses a 3beta-hydroxyl and 17-oxo function, estrone, estradiol, cortisol, 11beta-hydroxy-pregn-4-ene-3-20-dione, beta-naphthol, alpha-naphthylamine, bilirubin and diethylstilbestrol are no substrates for the purified enzyme Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.2 5
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.2 5 9
-
 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.8.2.2 5
-
 unstable at low pH Rattus norvegicus
2.8.2.2 6 9 stable for 30 min at 37°C Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.2 0.003
-
 adenosine 3',5'-bisphosphate pH 8.0, 37°C Rattus norvegicus