BRENDA - Enzyme Database show

Purification and characterization of human liver dehydroepiandrosterone sulphotransferase

Falany, C.N.; Vasquez, M.E.; Kalb, J.M.; Biochem. J. 260, 641-646 (1989)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.15
adenosine 3',5'-bisphosphate
-
Homo sapiens
2.8.2.15
pregnenolone
substrate inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.2.15
0.0016
-
3beta-hydroxyandrost-5-ene-17-one
pH 7.5, ?
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.2.15
cytosol
-
Homo sapiens
5829
-
2.8.2.15
endoplasmic reticulum
-
Homo sapiens
5783
-
2.8.2.15
Golgi apparatus
-
Homo sapiens
5794
-
2.8.2.15
membrane
-
Homo sapiens
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.15
68000
70000
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.2.15
Homo sapiens
-
human
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.2.15
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.15
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.2.15
0.133
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.15
3'-phosphoadenylylsulfate + 17beta-estradiol
estra-1,3,5(10)-triene-3,17alpha-diol
645729
Homo sapiens
adenosine 3',5'-bisphosphate + ?
-
-
-
r
2.8.2.15
3'-phosphoadenylylsulfate + 3alpha-hydroxy-5alpha-androstan-17-one
androsterone
645729
Homo sapiens
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
androsterone sulfate
645729
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + 3beta-hydroxy-5alpha-androstan-17-one
epiandrosterone
645729
Homo sapiens
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
-
645729
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + androst-5-ene-3beta,17beta-diol
-
645729
Homo sapiens
adenosine 3',5'-bisphosphate + ?
-
-
-
r
2.8.2.15
3'-phosphoadenylylsulfate + estrone
-
645729
Homo sapiens
adenosine 3',5'-bisphosphate + estrone sulfate
estra-1,3,5(10)-trien-17-one 3-sulfate
645729
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + etiocholanone
3alpha-hydroxy-5beta-androstan-17-one
645729
Homo sapiens
adenosine 3',5'-bisphosphate + etiocholanone sulfate
5beta-androstan-17-one 3-sulfate
645729
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + pregnenolone
-
645729
Homo sapiens
adenosine 3',5'-bisphosphate + pregnenolone sulfate
pregn-5-en-20-one 3-sulfate
645729
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + testosterone
17beta-hydroxyandrost-4-en-3-one
645729
Homo sapiens
adenosine 3',5'-bisphosphate + testosterone sulfate
androst-4-en-3-one 17-sulfate
645729
Homo sapiens
r
2.8.2.15
additional information
no activity towards p-nitrophenol or dopamine
645729
Homo sapiens
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.8.2.15
dimer
-
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.8.2.15
0.0016
-
adenosine 3',5'-bisphosphate
pH 7.5
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.15
adenosine 3',5'-bisphosphate
-
Homo sapiens
2.8.2.15
pregnenolone
substrate inhibition
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.8.2.15
0.0016
-
adenosine 3',5'-bisphosphate
pH 7.5
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.2.15
0.0016
-
3beta-hydroxyandrost-5-ene-17-one
pH 7.5, ?
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.2.15
cytosol
-
Homo sapiens
5829
-
2.8.2.15
endoplasmic reticulum
-
Homo sapiens
5783
-
2.8.2.15
Golgi apparatus
-
Homo sapiens
5794
-
2.8.2.15
membrane
-
Homo sapiens
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.15
68000
70000
gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.2.15
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.15
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.2.15
0.133
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.15
3'-phosphoadenylylsulfate + 17beta-estradiol
estra-1,3,5(10)-triene-3,17alpha-diol
645729
Homo sapiens
adenosine 3',5'-bisphosphate + ?
-
-
-
r
2.8.2.15
3'-phosphoadenylylsulfate + 3alpha-hydroxy-5alpha-androstan-17-one
androsterone
645729
Homo sapiens
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
androsterone sulfate
645729
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + 3beta-hydroxy-5alpha-androstan-17-one
epiandrosterone
645729
Homo sapiens
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
-
645729
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + androst-5-ene-3beta,17beta-diol
-
645729
Homo sapiens
adenosine 3',5'-bisphosphate + ?
-
-
-
r
2.8.2.15
3'-phosphoadenylylsulfate + estrone
-
645729
Homo sapiens
adenosine 3',5'-bisphosphate + estrone sulfate
estra-1,3,5(10)-trien-17-one 3-sulfate
645729
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + etiocholanone
3alpha-hydroxy-5beta-androstan-17-one
645729
Homo sapiens
adenosine 3',5'-bisphosphate + etiocholanone sulfate
5beta-androstan-17-one 3-sulfate
645729
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + pregnenolone
-
645729
Homo sapiens
adenosine 3',5'-bisphosphate + pregnenolone sulfate
pregn-5-en-20-one 3-sulfate
645729
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + testosterone
17beta-hydroxyandrost-4-en-3-one
645729
Homo sapiens
adenosine 3',5'-bisphosphate + testosterone sulfate
androst-4-en-3-one 17-sulfate
645729
Homo sapiens
r
2.8.2.15
additional information
no activity towards p-nitrophenol or dopamine
645729
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.8.2.15
dimer
-
Homo sapiens