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Literature summary extracted from

  • Takikawa, H.; Stolz, A.; Kaplowitz, N.
    Purification of a 32.5 kDa monomeric sulfotransferase from rat liver with activity for bile acids and phenolic steroids (1986), FEBS Lett., 207, 193-197.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.8.2.15 marked instability, addition of 20% glycerol improves stability Rattus norvegicus
2.8.2.34 20% glycerol stabilizes Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.34 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.34 Mg2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.15 32500
-
-
 Rattus norvegicus
2.8.2.15 60000 70000 gel filtration Rattus norvegicus
2.8.2.34 32500
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.15 Rattus norvegicus
-
 rat
-
2.8.2.15 Rattus norvegicus
-
 Sprague-Dawley
-
2.8.2.15 Rattus norvegicus
-
 male rats
-
2.8.2.15 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-
2.8.2.34 Rattus norvegicus
-
 male sprague-dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.15
-
 Rattus norvegicus
2.8.2.34 from liver, by gel filtration, affinity chromatography, chromatofocusing, and hydroxyapatite chromatography, to homogeneity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.15 liver
-
 Rattus norvegicus
-
2.8.2.34 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.15 0.000831
-
-
 Rattus norvegicus
2.8.2.34 0.000094
-
 purified enzyme, substrate glycolithocholate Rattus norvegicus
2.8.2.34 0.000831
-
 purified enzyme, substrate estradiol Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.15 3'-phosphoadenylyl sulfate + taurolithocholate
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + taurolithocholate 3-sulfate
-
 r
2.8.2.15 3'-phosphoadenylyl sulfate + taurolithocholate
-
 Rattus norvegicus Sprague-Dawley adenosine 3',5'-bisphosphate + taurolithocholate 3-sulfate
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + 17beta-estradiol estra-1,3,5(10)-triene-3,17alpha-diol Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + 17beta-estradiol estra-1,3,5(10)-triene-3,17alpha-diol Rattus norvegicus Sprague-Dawley adenosine 3',5'-bisphosphate + ?
-
 r
2.8.2.15 additional information activity for bile acids and phenolic steroids Rattus norvegicus ?
-
 ?
2.8.2.15 additional information activity for bile acids and phenolic steroids Rattus norvegicus Sprague-Dawley ?
-
 ?
2.8.2.34 3'-phosphoadenylyl 5'-phosphosulfate + estradiol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.34 3'-phosphoadenylyl 5'-phosphosulfate + estradiol
-
 Rattus norvegicus Sprague-Dawley adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.34 3'-phosphoadenylyl 5'-phosphosulfate + glycolithocholate
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
-
 ?
2.8.2.34 3'-phosphoadenylyl 5'-phosphosulfate + glycolithocholate
-
 Rattus norvegicus Sprague-Dawley adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
-
 ?
2.8.2.34 3'-phosphoadenylyl 5'-phosphosulfate + taurolithocholate
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + taurolithocholate 3-sulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.15 monomer 1 * 32500, SDS-PAGE Rattus norvegicus
2.8.2.34 monomer 1 * 32500, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.34 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.34 7
-
 assay at Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.2.34 Rattus norvegicus chromatofocusing
-
 5.1