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Literature summary extracted from

  • Adams, J.B.; McDonald, D.
    Enzymic synthesis of steroid sulphates. XII. Isolation of dehydroepiandrosterone sulphotransferase from human adrenals by affinity chromatography (1979), Biochim. Biophys. Acta, 567, 144-153.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.15 cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.15 34500
-
 2 * 34500, SDS-PAGE Homo sapiens
2.8.2.15 35000
-
 2 * 35000, SDS-PAGE Homo sapiens
2.8.2.15 35000
-
 2 * 35000, amino acid analysis Homo sapiens
2.8.2.15 68000
-
 sucrose density gradient ultracentrifugation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.15 Homo sapiens
-
 human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.15
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.15 adrenal cortex
-
 Homo sapiens
-
2.8.2.15 adrenal gland
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.15 0.0073
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.8.2.15 0°C, redialyzed againstTris/thiol buffer, stored under N2, activity retains for at least 4 weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.15 3'-phosphoadenylylsulfate + 17beta-estradiol estra-1,3,5(10)-triene-3,17alpha-diol Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + 3alpha-hydroxy-5alpha-androstan-17-one androsterone Homo sapiens adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate androsterone sulfate r
2.8.2.15 3'-phosphoadenylylsulfate + 3beta-hydroxy-5alpha-androstan-17-one epiandrosterone Homo sapiens adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + androst-5-ene-3beta17alpha-diol
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + cholesterol sulfurylated at low rate Homo sapiens adenosine 3',5'-bisphosphate + cholesterol sulfate
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + etiocholanone 3alpha-hydroxy-5beta-androstan-17-one Homo sapiens adenosine 3',5'-bisphosphate + etiocholanone sulfate 5beta-androstan-17-one 3-sulfate r
2.8.2.15 3'-phosphoadenylylsulfate + pregnenolone
-
 Homo sapiens adenosine 3',5'-bisphosphate + pregnenolone sulfate pregn-5-en-20-one 3-sulfate r
2.8.2.15 3'-phosphoadenylylsulfate + testosterone 17beta-hydroxyandrost-4-en-3-one Homo sapiens adenosine 3',5'-bisphosphate + testosterone sulfate androst-4-en-3-one 17-sulfate r

Subunits

EC Number Subunits Comment Organism
2.8.2.15 dimer 2 * 35000, SDS-PAGE Homo sapiens
2.8.2.15 dimer 2 * 34500, SDS-PAGE Homo sapiens
2.8.2.15 dimer 2 * 35000, amino acid analysis Homo sapiens