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Literature summary extracted from

  • Singer, S.S.; Gebhart, J.; Hess, E.
    Enzymatic sulfation of steroids. V. Partial purification and some properties of sulfotransferase III, the major glucocorticoid sulfotransferase of liver cytosol from male rats (1978), Can. J. Biochem., 56, 1028-1035.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.18 additional information no activation by adenine nucleotides Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.15 Cd2+ strongly inhibited by 5 mM Rattus norvegicus
2.8.2.15 corticosterone competitive inhibitor, inhibits cortisol sulfotransferase activity Rattus norvegicus
2.8.2.15 cortisol
-
 Rattus norvegicus
2.8.2.15 cortisone
-
 Rattus norvegicus
2.8.2.15 dehydroepiandrosterone competitive inhibitor, inhibits cortisol sulfotransferase activity Rattus norvegicus
2.8.2.15 deoxycorticosterone competitive inhibitor, inhibits cortisol sulfotransferase activity Rattus norvegicus
2.8.2.15 estradiol-17beta competitive inhibitor, inhibits cortisol sulfotransferase activity Rattus norvegicus
2.8.2.15 additional information unaffected by 8 mM ADP, ATP, and AMP, diethylstilbestrol, cortisone and cholesterol are no inhibitors for the purified enzyme Rattus norvegicus
2.8.2.15 p-hydroxymercuribenzoate
-
 Rattus norvegicus
2.8.2.15 progesterone competitive inhibitor, inhibits cortisol sulfotransferase activity Rattus norvegicus
2.8.2.15 testosterone competitive inhibitor, inhibits cortisol sulfotransferase activity Rattus norvegicus
2.8.2.15 Zn2+ strongly inhibited by 5 mM Rattus norvegicus
2.8.2.18 Cd2+ 5 mM, strong inhibition Rattus norvegicus
2.8.2.18 dehydroepiandrosterone cortisol as substrate; kinetics Rattus norvegicus
2.8.2.18 dexamethasone cortisol as substrate; kinetics Rattus norvegicus
2.8.2.18 diethylstilbestrol cortisol as substrate Rattus norvegicus
2.8.2.18 additional information no inhibition by adenine nucleotides Rattus norvegicus
2.8.2.18 progesterone cortisol as substrate Rattus norvegicus
2.8.2.18 Zn2+ 5 mM, strong inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.15 0.00628
-
 3'-phosphoadenylylsulfate pH 6.8, 37.5°C Rattus norvegicus
2.8.2.15 0.00682
-
 cortisol pH 6.8, 37.5°C Rattus norvegicus
2.8.2.18 0.00628
-
 3'-phosphoadenylylsulfate pH 6.8, 37.5°C Rattus norvegicus
2.8.2.18 0.00682
-
 cortisol pH 6.8, 37.5°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.15 cytoplasm
-
 Rattus norvegicus 5737
-
2.8.2.15 cytosol
-
 Rattus norvegicus 5829
-
2.8.2.18 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.15 Ba2+ markedly activated by 5 mM divalent cations Rattus norvegicus
2.8.2.15 Ca2+ markedly activated by 5 mM divalent cations Rattus norvegicus
2.8.2.15 Co2+ markedly activated by 5 mM divalent cations Rattus norvegicus
2.8.2.15 Cr2+ markedly activated by 5 mM divalent cations Rattus norvegicus
2.8.2.15 Mg2+ markedly activated by 5 mM divalent cations Rattus norvegicus
2.8.2.15 Mn2+ most effective, markedly activated by 5 mM divalent cations Rattus norvegicus
2.8.2.15 Ni2+ markedly activated by 5 mM divalent cations Rattus norvegicus
2.8.2.18 Ba2+ activation Rattus norvegicus
2.8.2.18 Ba2+ 5 mM Rattus norvegicus
2.8.2.18 Ba2+ in decreasing order of efficiency: Mn2+, Cr2+, Co2+, Mg2+, Ba2+, Ca2+, Ni2+ Rattus norvegicus
2.8.2.18 Ca2+ activation Rattus norvegicus
2.8.2.18 Ca2+ 5 mM Rattus norvegicus
2.8.2.18 Ca2+ in decreasing order of efficiency: Mn2+, Cr2+, Co2+, Mg2+, Ba2+, Ca2+, Ni2+ Rattus norvegicus
2.8.2.18 Co2+ activation Rattus norvegicus
2.8.2.18 Co2+ 5 mM Rattus norvegicus
2.8.2.18 Co2+ in decreasing order of efficiency: Mn2+, Cr2+, Co2+, Mg2+, Ba2+, Ca2+, Ni2+ Rattus norvegicus
2.8.2.18 Cr2+ activation Rattus norvegicus
2.8.2.18 Cr2+ 5 mM Rattus norvegicus
2.8.2.18 Cr2+ in decreasing order of efficiency: Mn2+, Cr2+, Co2+, Mg2+, Ba2+, Ca2+, Ni2+ Rattus norvegicus
2.8.2.18 Mg2+ activation Rattus norvegicus
2.8.2.18 Mg2+ 5 mM Rattus norvegicus
2.8.2.18 Mg2+ in decreasing order of efficiency: Mn2+, Cr2+, Co2+, Mg2+, Ba2+, Ca2+, Ni2+ Rattus norvegicus
2.8.2.18 Mn2+ activation Rattus norvegicus
2.8.2.18 Mn2+ 5 mM Rattus norvegicus
2.8.2.18 Mn2+ in decreasing order of efficiency: Mn2+, Cr2+, Co2+, Mg2+, Ba2+, Ca2+, Ni2+ Rattus norvegicus
2.8.2.18 Ni2+ activation Rattus norvegicus
2.8.2.18 Ni2+ 5 mM Rattus norvegicus
2.8.2.18 Ni2+ in decreasing order of efficiency: Mn2+, Cr2+, Co2+, Mg2+, Ba2+, Ca2+, Ni2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.15 61500
-
 gel filtration Rattus norvegicus
2.8.2.15 65000
-
 sedimentation velocity experiment in preparative ultracentrifuge Rattus norvegicus
2.8.2.18 61500
-
 sulfotransferase III, gel filtration Rattus norvegicus
2.8.2.18 65000
-
 sedimentation velocity experiments Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.15 3'-phosphoadenylylsulfate + dehydroepiandrosterone Rattus norvegicus enzymatic sulfation of steroids adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r
2.8.2.18 3'-phosphoadenylylsulfate + cortisol Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + cortisol 21-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.15 Rattus norvegicus
-
 rat
-
2.8.2.15 Rattus norvegicus
-
 CDR Fisher, male
-
2.8.2.18 Rattus norvegicus
-
 male CDR Fisher rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.15 partial Rattus norvegicus
2.8.2.18 partial Rattus norvegicus
2.8.2.18 sulfotransferase III Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.15 liver
-
 Rattus norvegicus
-
2.8.2.18 blood
-
 Rattus norvegicus
-
2.8.2.18 liver
-
 Rattus norvegicus
-
2.8.2.18 additional information tissue distribution Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.15 0.000978
-
-
 Rattus norvegicus
2.8.2.18 0.00098
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.8.2.15 -20°C, partially purified enzyme, stable for at least 3 months Rattus norvegicus
2.8.2.18 -20°C, at least 3 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.15 3'-phosphoadenylylsulfate + 17beta-estradiol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + cortisol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + cortisol sulfate
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + dehydroepiandrosterone enzymatic sulfation of steroids Rattus norvegicus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + testosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + testosterone sulfate
-
 r
2.8.2.18 3'-phosphoadenylylsulfate + cortisol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + cortisol 21-sulfate
-
 ?
2.8.2.18 3'-phosphoadenylylsulfate + cortisol i.e. PAPS, glucocorticoids are preferred substrates Rattus norvegicus adenosine 3',5'-bisphosphate + cortisol 21-sulfate
-
 ?
2.8.2.18 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.15 STIII
-
 Rattus norvegicus
2.8.2.15 sulfotransferase III
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.18 37.5
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.15 6
-
-
 Rattus norvegicus
2.8.2.18 6
-
 cortisol as substrate Rattus norvegicus