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Literature summary extracted from

  • Tennekoon, G.; Aitchinson, S.; Zabura, M.
    Purification and characterization of galactocerebroside sulfotransferase from rat kidney (1985), Arch. Biochem. Biophys., 240, 932-944.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.11 2-Deoxy-3'-phosphoadenosine 5'-phosphate
-
 Rattus norvegicus
2.8.2.11 3'-phosphoadenosine 5'-phosphate
-
 Rattus norvegicus
2.8.2.11 guanosine 3',5'-diphosphate
-
 Rattus norvegicus
2.8.2.11 Mg2+ 1-10 mM: enhances activity, 20-35 mM: inhibition Rattus norvegicus
2.8.2.11 Mn2+ 1-10 mM: enhances activity, 20-35 mM: inhibition Rattus norvegicus
2.8.2.11 additional information not: 2'-phosphoadenosine 5'-phosphate, fluorosulfonylbenzoyladenosine Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.11 0.0052
-
 3'-phosphoadenylylsulfate pH 6.2, 37°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.11 Ca2+ 1-10 mM and 20-35 mM: enhancement Rattus norvegicus
2.8.2.11 Mg2+ 1-10 mM: enhances activity, 20-35 mM: inhibition Rattus norvegicus
2.8.2.11 Mn2+ 1-10 mM: enhances activity, 20-35 mM: inhibition Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.11 64000
-
 nondenaturing PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.11 additional information Rattus norvegicus enzyme regulates the sulfation of glycolipids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.11 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.8.2.11 additional information purified enzyme contains bound lipids, consisting primarily of cholesterol and phosphatidylcholine, removal of associated lipids results in loss of activity Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.11
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.11 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.11 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.8.2.11 -20°C, 50% glycerol, 0.1% Triton X-100, pH 7.0-7.5, stable for more than 4 weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.11 galactosylceramide + 3'-phosphoadenylylsulfate biotinylated and light-sensitive azido derivatives of lysogalactosylceramide are synthesized by the crude enzyme preparation, these derivatives remain effective substrates for the testicular enzyme Rattus norvegicus galactosylceramide sulfate + adenosine 3',5'-bisphosphate
-
 ?
2.8.2.11 additional information enzyme regulates the sulfation of glycolipids Rattus norvegicus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.2.11 60
-
 10 min, complete loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.11 5.8 6
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.11 0.034
-
 guanosine 3',5'-diphosphate pH 6.2, 37°C Rattus norvegicus
2.8.2.11 0.3
-
 3'-phosphoadenosine 5'-phosphate pH 6.2, 37°C Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.2.11 Rattus norvegicus isoelectric foccusing
-
 5.1