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Literature summary extracted from

  • Sundaram, K.S.; Lev, M.
    Purification and activation of brain sulfotransferase (1992), J. Biol. Chem., 267, 24041-24044.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.11 ATP
-
 Mus musculus
2.8.2.11 vitamin K1 with phosphate, activates purified preparation Mus musculus

General Stability

EC Number General Stability Organism
2.8.2.11 ethylene glycol, 2-mercaptoethanol, 10 mM ATP and a mixture of phosphatidylcholine and phosphatidylethanolamine ratio 1:1 does not improve stability at -80°C Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.11 0.0012
-
 3'-phosphoadenylylsulfate pH 7.0, 37°C Mus musculus
2.8.2.11 0.026
-
 cerebroside pH 7.0, 37°C Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.11 28000
-
 gel filtration Mus musculus
2.8.2.11 31000
-
 1 * 31000 SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.11 galactocerebroside + 3'-phosphoadenylylsulfate Mus musculus enzyme catalyses the biosynthesis of sulfatides, sulfatides are important components of myelin sulfogalactoglycerolipid + adenosine 3',5'-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.11 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.11
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.11 brain
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.11 additional information
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.8.2.11 -80°C, 35% loss of activity after 7 days Mus musculus
2.8.2.11 -80°C, addition of vitamin K + phosphate, stable for 14 days Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.11 3'-phosphoadenylyl sulfate + a galactosylceramide
-
 Mus musculus adenosine 3',5'-bisphosphate + a galactosylceramidesulfate
-
 ?
2.8.2.11 3'-phosphoadenylyl sulfate + cerebroside
-
 Mus musculus adenosine 3',5'-bisphosphate + cerebroside 3-sulfate
-
 ?
2.8.2.11 galactocerebroside + 3'-phosphoadenylylsulfate
-
 Mus musculus galactocerebroside 3-sulfate + adenosine 3',5'-bisphosphate
-
 ?
2.8.2.11 galactocerebroside + 3'-phosphoadenylylsulfate enzyme catalyses the biosynthesis of sulfatides, sulfatides are important components of myelin Mus musculus sulfogalactoglycerolipid + adenosine 3',5'-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.11 monomer 1 * 31000 SDS-PAGE Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.2.11 45
-
 10 min, complete loss of activity Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.11 7
-
-
 Mus musculus