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Literature summary extracted from

  • Liu, M.C.; Suiko, M.; Sakakibara, Y.
    Mutational analysis of the substrate binding/catalytic domains of human M form and P form phenol sulfotransferases (2000), J. Biol. Chem., 275, 13460-13464.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.1 expression of wild-type P-PST and M-PST and diverse mutants of both in Escherichia coli XL1-Blue Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.1 A146E P-PST mutant by site-directed mutagenesis, highly reduced activity with dopamine, highly increased activity wih 4-nitrophenol Homo sapiens
2.8.2.1 A86D/I89E P-PST mutant by site-directed mutagenesis, highly reduced activity with dopamine, highly increased activity wih 4-nitrophenol Homo sapiens
2.8.2.1 A86D/I89E/A146E P-PST mutant by site-directed mutagenesis, no activity Homo sapiens
2.8.2.1 D86A M-PST mutant by site-directed mutagenesis, reduced activity Homo sapiens
2.8.2.1 D86A/E89I M-PST mutant by site-directed mutagenesis, reduced activity Homo sapiens
2.8.2.1 D86A/E89I/E146A M-PST mutant by site-directed mutagenesis, highly reduced activity with dopamine, and highly increased activity with 4-nitrophenol Homo sapiens
2.8.2.1 E146A M-PST mutant by site-directed mutagenesis, highly reduced activity with dopamine, and highly increased activity with 4-nitrophenol Homo sapiens
2.8.2.1 E89I M-PST mutant by site-directed mutagenesis, reduced activity Homo sapiens
2.8.2.1 H108A P-PST mutant by site-directed mutagenesis, no activity Homo sapiens
2.8.2.1 H108A M-PST mutant by site-directed mutagenesis, no activity with 4-nitrophenol, highly reduced activity with dopamine Homo sapiens
2.8.2.1 H143Y M-PST mutant by site-directed mutagenesis, reduced activity with dopamine, unaltered activity wih 4-nitrophenol Homo sapiens
2.8.2.1 I89E P-PST mutant by site-directed mutagenesis, highly reduced activity with dopamine, highly increased activity wih 4-nitrophenol Homo sapiens
2.8.2.1 I89E/A146E P-PST mutant by site-directed mutagenesis, highly reduced activity with dopamine, no activity wih 4-nitrophenol Homo sapiens
2.8.2.1 K48M P-PST and M-PST mutants by site-directed mutagenesis, no activity Homo sapiens
2.8.2.1 K48M/H108A M-PST mutant by site-directed mutagenesis, no activity Homo sapiens
2.8.2.1 N85K M-PST mutant by site-directed mutagenesis, reduced activity Homo sapiens
2.8.2.1 N85K/D86A M-PST mutant by site-directed mutagenesis, reduced activity with dopamine, unaltered activity wih 4-nitrophenol Homo sapiens
2.8.2.1 N85K/E89I M-PST mutant by site-directed mutagenesis, reduced activity Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Homo sapiens
-
 2 forms: simple phenol sulfating P-PST, monoamine sulfating M-PST
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + a phenol
-
 Homo sapiens adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?