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Literature summary extracted from

  • Sakakibara, Y.; Yanagisawa, K.; Takami, Y.; Nakayama, T.; Suiko, M.; Liu, M.C.
    Molecular cloning, expression, and functional characterization of novel mouse sulfotransferases (1998), Biochem. Biophys. Res. Commun., 247, 681-686.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.1 expression of wild-type P-PST and M-PST and chimeric mutants bearing parts of both enzyme forms in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.1 additional information construction of several chimeric mutants by exchange of sequence fragments between the P-PST and the M-PST form, the structural determinants for the substrate specificity of the 2 forms comprsie the internal domain-spanning amino acid residues 84-148 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.1 additional information
-
 additional information kinetics, chimeric enzymes Homo sapiens
2.8.2.1 0.00036
-
 4-nitrophenol recombinant wild-type P-PST, pH 7.0, 37°C Homo sapiens
2.8.2.1 0.0014
-
 dopamine recombinant wild-type M-PST, pH 7.0, 37°C Homo sapiens
2.8.2.1 0.0875
-
 dopamine recombinant wild-type P-PST, pH 7.0, 37°C Homo sapiens
2.8.2.1 0.167
-
 4-nitrophenol recombinant wild-type M-PST, pH 7.0, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.1 Mn2+ stimulates M-PST about 3.5fold, with substrate D-tyrosine Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Homo sapiens O43704 2 forms: simple phenol sulfating P-PST, monoamine sulfating M-PST
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.1 recombinant wild-type P-PST and M-PST and chimeric mutants Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + a phenol
-
 Homo sapiens adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 4-nitrophenol M-PST Homo sapiens adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 4-nitrophenol P-PST Homo sapiens adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + D-3,4-dihydroxyphenylalanine M-PST Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + D-3,4-dihydroxyphenylalanine i.e. D-Dopa Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + D-tyrosine M-PST Homo sapiens adenosine 3',5'-bisphosphate + D-tyrosyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + DL-tyrosine M-PST Homo sapiens adenosine 3',5'-bisphosphate + DL-tyrosyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + dopamine M-PST Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + dopamine P-PST Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + L-3,4-dihydroxyphenylalanine i.e. L-dopa Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + L-3,4-dihydroxyphenylalanine M-PST Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + L-tyrosine M-PST Homo sapiens adenosine 3',5'-bisphosphate + L-tyrosyl O4-sulfate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.1 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.1 7
-
 assay at Homo sapiens