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Literature summary extracted from

  • Yang, Y.S.; Tsai, S.W.; Lin, E.S.
    Effects of 3'-phosphoadenosine 5'-phosphate on the activity and folding of phenol sulfotransferase (1998), Chem. Biol. Interact., 109, 129-135.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.1 overexpression of alpha- and beta-enzyme form in Escherichia coli, at high expression levels, the beta-enzyme form becomes the majority, while the alpha-enzyme form increases only slightly compared to low level expression Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Rattus norvegicus
-
 alpha- and beta-enzyme form
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.2.1 3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate the overall physiological reaction is a transfer of the sulfate group between 2-naphthol and 4-nitrophenol with the intermediate formation of 3'-phosphoadenylyl sulfate Rattus norvegicus
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.8.2.1 the alpha-enzyme form renatures in presence of adenosine 3',5'-bisphosphate, while the beta-enzyme form renature only in absence of adenosine 3',5'-bisphosphate Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + a phenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 2-naphthol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 r
2.8.2.1 3'-phosphoadenylylsulfate + 4-nitrophenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 r

Subunits

EC Number Subunits Comment Organism
2.8.2.1 More alpha- and beta-enzyme forms assemble through different folding processes Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.1 7
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.2.1 3'-phosphoadenylyl sulfate the overall physiological reaction is a transfer of the sulfate group between 2-naphthol and 4-nitrophenol with the intermediate formation of 3'-phosphoadenylyl sulfate from adenosine 3',5'-bisphosphate Rattus norvegicus
2.8.2.1 adenosine 3',5'-bisphosphate the overall physiological reaction is a transfer of the sulfate group between 2-naphthol and 4-nitrophenol with the intermediate formation of 3'-phosphoadenylyl sulfate from adenosine 3',5'-bisphosphate Rattus norvegicus