Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sekura, R.D.; Jakoby, W.B.
    Phenol sulfotransferases (1979), J. Biol. Chem., 254, 5658-5663.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.1 GSH increases activity Rattus norvegicus

General Stability

EC Number General Stability Organism
2.8.2.1 glycerol reduces activity Rattus norvegicus
2.8.2.1 sucrose and 2-mercaptoethanol stabilize during purification Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.1 adenosine 3',5'-bisphosphate
-
 Rattus norvegicus
2.8.2.1 ADP isoenzyme I Rattus norvegicus
2.8.2.1 ATP isoenzyme I Rattus norvegicus
2.8.2.1 Cibacron blue isozyme I Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.1 0.0065
-
 3'-phosphoadenylylsulfate with 2-naphthol, isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.012
-
 3'-phosphoadenylylsulfate with 2-naphthol, isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.06
-
 2-naphthol isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.07
-
 3-hydroxyindole isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.08
-
 3-hydroxyindole isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.09
-
 2-naphthol isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.12
-
 3-chlorophenol isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.15
-
 2-chlorophenol isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.16
-
 3-chlorophenol isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.18
-
 4-Hydroxybiphenyl isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.25
-
 4-Hydroxybiphenyl isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.29
-
 5-hydroxyindole isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.3
-
 5-hydroxyindole isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.44
-
 3-Nitrophenol isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.53
-
 5-hydroxytryptamine isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 0.6
-
 hydroquinone isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 1
-
 3-Nitrophenol isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 1.2
-
 4-Chlorophenol isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 1.4
-
 3-methylphenol isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 1.5
-
 4-Chlorophenol isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 1.6
-
 4-nitrophenol isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 1.6
-
 5-hydroxytryptamine isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 1.6
-
 4-acetamidophenol isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 1.8
-
 phenol isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 1.8
-
 hydroquinone isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 2.1
-
 4-acetamidophenol isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 2.2
-
 4-Methylphenol isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 2.5
-
 4-nitrophenol isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 2.6
-
 phenol isoenzyme II, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 2.9
-
 4-Methylphenol isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 4.2
-
 4-methoxyphenol isoenzyme I, pH 6.5, 37°C Rattus norvegicus
2.8.2.1 6.5
-
 4-methoxyphenol isoenzyme II, pH 6.5, 37°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.1 35000
-
 2 * 35000, SDS-PAGE Rattus norvegicus
2.8.2.1 64000 65000 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.1 3'-phosphoadenylylsulfate + 3-hydroxyindole Rattus norvegicus in vivo function may include the production of the normal tryptophan metabolite indican adenosine 3',5'-bisphosphate + 3-indoxyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + a phenol Rattus norvegicus enzyme may be considered as detoxification enzyme which catalyzes the conjugation of xenobiotics containing a phenol group or of phenolic compounds generated by endogenous oxidation adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.1 isoenzyme I and II Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.1 0.282
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.8.2.1 -80°C, storage over night, 10%, transferase I, and 35%, transferase II, loss of activity Rattus norvegicus
2.8.2.1 4°C, stable for at least 2 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + 2,6-dichloro-4-nitrophenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 2,6-dichloro-4-nitrophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + a phenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 2-chlorophenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 2-chlorophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 2-naphthol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 3-chlorophenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 3-chlorophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 3-hydroxyindole
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 3-indoxyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 3-hydroxyindole in vivo function may include the production of the normal tryptophan metabolite indican Rattus norvegicus adenosine 3',5'-bisphosphate + 3-indoxyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 3-methylphenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 3-methylphenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 3-nitrophenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 3-nitrophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 4-acetamidophenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 4-acetamidophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 4-chlorophenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 4-chlorophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 4-methoxyphenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 4-methoxyphenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 4-methylphenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 4-methylphenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 5-hydroxyindole
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 5-indoxyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 5-hydroxytryptamine
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 3-(2-aminoethyl)-5-indoxyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + a phenol enzyme may be considered as detoxification enzyme which catalyzes the conjugation of xenobiotics containing a phenol group or of phenolic compounds generated by endogenous oxidation Rattus norvegicus adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + dopamine
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + hydroquinone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + hydroxybiphenyl
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + phenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + phenyl sulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.1 dimer 2 * 35000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.1 37
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.2.1 additional information
-
 additional information
-
 Rattus norvegicus
2.8.2.1 0.01
-
 5-hydroxytryptamine isoenzyme II Rattus norvegicus
2.8.2.1 0.025
-
 5-hydroxytryptamine isoenzyme I Rattus norvegicus
2.8.2.1 0.113
-
 phenol isoenzyme I Rattus norvegicus
2.8.2.1 0.122
-
 phenol isoenzyme II Rattus norvegicus
2.8.2.1 0.6
-
 5-hydroxyindole isoenzyme II Rattus norvegicus
2.8.2.1 0.65
-
 3-hydroxyindole
-
 Rattus norvegicus
2.8.2.1 0.65
-
 5-hydroxyindole isoenzyme I Rattus norvegicus
2.8.2.1 0.733
-
 2-chlorophenol isoenzyme I Rattus norvegicus
2.8.2.1 0.733
-
 3-hydroxyindole isoenzyme I Rattus norvegicus
2.8.2.1 0.783
-
 2-naphthol isoenzyme I Rattus norvegicus
2.8.2.1 0.9
-
 2-naphthol isoenzyme II Rattus norvegicus
2.8.2.1 1.55
-
 4-methoxyphenol isoenzyme I Rattus norvegicus
2.8.2.1 2
-
 4-methoxyphenol isoenzyme II Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.1 6.5
-
 with substrate 4-nitrophenol Rattus norvegicus
2.8.2.1 6.5
-
 with substrate 2-naphthol, a second optimum at pH 9.5 Rattus norvegicus
2.8.2.1 9.5
-
 substrate 2-naphthol, second optimum at pH 6.5 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.1 additional information
-
-
 Rattus norvegicus