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Literature summary extracted from

  • Romain, Y.; Demassieux, S.; Carriere, S.
    Partial purification and characterization of two isoenzymes involved in the sulfurylation of catecholamines (1982), Biochem. Biophys. Res. Commun., 106, 999-1005.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.1 EDTA 10 mM, stimulates isozymes A and B Canis lupus familiaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.1 Ca2+
-
 Canis lupus familiaris
2.8.2.1 Cu2+
-
 Canis lupus familiaris
2.8.2.1 KCl 50 mM, 60% inhibition of isoenzyme A, slight activation of isoenzyme B Canis lupus familiaris
2.8.2.1 Mg2+
-
 Canis lupus familiaris
2.8.2.1 Zn2+
-
 Canis lupus familiaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.1 0.0017
-
 3'-phosphoadenylylsulfate with dopamine, isoenzyme A, pH 6.0, 37°C Canis lupus familiaris
2.8.2.1 0.0062
-
 dopamine isoenzyme B, pH 9.5, 37°C Canis lupus familiaris
2.8.2.1 0.0177
-
 dopamine isoenzyme A, pH 6.0, 37°C Canis lupus familiaris
2.8.2.1 0.026
-
 3'-phosphoadenylylsulfate with dopamine, isoenzyme B, pH 9.5, 37°C Canis lupus familiaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.1 KCl
-
 Canis lupus familiaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.1 60000
-
 gel filtration Canis lupus familiaris

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Canis lupus familiaris
-
 isozymes A and B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.1 isozyme A 18fold, isozyme B 35fold Canis lupus familiaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.1 liver
-
 Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.1 0.0016
-
 purified isozyme A Canis lupus familiaris
2.8.2.1 0.0036
-
 purified isozyme B Canis lupus familiaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + a phenol
-
 Canis lupus familiaris adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 2-hydroxyestradiol
-
 Canis lupus familiaris adenosine 3',5'-bisphosphate + estradiol 2-sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 2-hydroxyestrone
-
 Canis lupus familiaris adenosine 3',5'-bisphosphate + estrone 2-sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 4-nitrophenol
-
 Canis lupus familiaris adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + adrenaline
-
 Canis lupus familiaris adenosine 3',5'-bisphosphate + adrenaline sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + dopamine
-
 Canis lupus familiaris adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + norepinephrine i.e. noradrenalin Canis lupus familiaris adenosine 3',5'-bisphosphate + norepinephrine sulfate i.e. noradrenalin sulfate ?
2.8.2.1 additional information isoenzyme B catalyzes the sulfurylation of a wider range of substrates than A which is preferentially active with dopamine Canis lupus familiaris ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.1 37
-
 assay at Canis lupus familiaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.1 5.5
-
 with substrate 4-nitrophenol, isoenzyme B Canis lupus familiaris
2.8.2.1 6
-
 with substrate dopamine, isoenzyme A Canis lupus familiaris
2.8.2.1 6.5
-
 isoenzyme A Canis lupus familiaris
2.8.2.1 6.5
-
 with substrate 4-nitrophenol Canis lupus familiaris
2.8.2.1 9.5
-
 with substrate dopamine, isoenzyme B Canis lupus familiaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.1 additional information
-
-
 Canis lupus familiaris