Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mihara, H.; Kurihara, T.; Yoshimura, T.; Esaki, N.
    Kinetic and mutational studies of three NifS homologs from Escherichia coli: mechanistic difference between L-cysteine desulfurase and L-selenocysteine lyase reactions (2000), J. Biochem., 127, 559-567.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.1.7 pyruvate increases activity of CSD towards L-selenocysteine, but not towards L-cysteine Escherichia coli
4.4.1.16 pyruvate
-
 Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.16
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.7 C328A IscS mutant, activity towards L-cysteine is almost completely abolished, activity toward L-selenocysteine is much less affected Escherichia coli
2.8.1.7 C358A CSD mutant, activity towards L-cysteine is almost completely abolished, activity toward L-selenocysteine is much less affected Escherichia coli
2.8.1.7 C364A CsdB mutant, activity towards L-cysteine is almost completely abolished, activity toward L-selenocysteine is much less affected Escherichia coli
4.4.1.16 C364A increased activity towards L-selenocysteine and L-cysteine sulfinate, activity toward L-cysteine was completely abolished Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.7 1.1
-
 L-selenocysteine pH 8, IcsS in the presence of pyruvate Escherichia coli
2.8.1.7 1.4
-
 L-selenocysteine pH 8, CSD in the presence of pyruvate Escherichia coli
2.8.1.7 2.6
-
 L-selenocysteine pH 8, CsdB in the presence of pyruvate Escherichia coli
2.8.1.7 3.5
-
 L-cysteine sulfinic acid pH 8, CSD in the presence of pyruvate Escherichia coli
2.8.1.7 26
-
 L-cysteine sulfinic acid pH 8, IcsS in the absence of pyruvate Escherichia coli
2.8.1.7 30
-
 L-cysteine sulfinic acid pH 8, CsdB in the presence of pyruvate Escherichia coli
2.8.1.7 39
-
 L-cysteine sulfinic acid pH 8, CsdB in the absence of pyruvate Escherichia coli
2.8.1.7 190
-
 L-cysteine sulfinic acid pH 8, IcsS in the presence of pyruvate Escherichia coli
4.4.1.16 2.6
-
 L-selenocysteine in the presence of pyruvate, pH 7.5 Escherichia coli
4.4.1.16 30
-
 L-cysteine sulfinate in the presence of pyruvate, pH 7.5 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.7 L-cysteine + [enzyme]-cysteine Escherichia coli
-
 L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Escherichia coli
-
 selenide + L-alanine + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.7 Escherichia coli
-
-
-
4.4.1.16 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.7 three homologs: CSD, CsdB and IscS Escherichia coli
4.4.1.16 overexpressed protein Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.7 L-cysteine + [enzyme]-cysteine
-
 Escherichia coli L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
2.8.1.7 L-cysteine sulfinic acid
-
 Escherichia coli L-alanine + sulfite
-
 ?
2.8.1.7 L-selenocysteine
-
 Escherichia coli L-alanine + selenium
-
 ?
4.4.1.16 L-cysteine sulfinic acid + reduced acceptor
-
 Escherichia coli SO2 + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Escherichia coli selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor enzyme bound pyridoxal 5'-phosphate serves as acceptor Escherichia coli selenide + L-alanine + acceptor
-
 ?
4.4.1.16 additional information traces of activity with L-cysteine Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.16 More two other proteins (CDS and IscS) with activity towards L-selenocysteine identified that are much less specific Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.16 pyridoxal 5'-phosphate required Escherichia coli