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Literature summary extracted from

  • Mihara, H.; Fujii, T.; Kato, S.; Kurihara, T.; Hata, Y.; Esaki, N.
    Structure of external aldimine of Escherichia coli CsdB, an IscS/NifS homolog: implications for its specificity toward selenocysteine (2002), J. Biochem., 131, 679-685.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.16
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.1.7 hanging drop vapor diffusion method, complexed with L-propargylglycine Escherichia coli
4.4.1.16 hanging drop vapor diffusion method Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.7 H123A decreased specific activity towards L-selenocysteine Escherichia coli
2.8.1.7 H55A normal activity towards L-selenocysteine and L-cysteine Escherichia coli
2.8.1.7 R379A significant loss of activity towards L-selenocysteine Escherichia coli
4.4.1.16 H123A only 1% of activity remaining compared to wild type, contributes to stabilization of pyridoxal 5'-phospahte Escherichia coli
4.4.1.16 H55A no effect on activity Escherichia coli
4.4.1.16 R379A less than 1% of activity remaining compared to wild type Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.7 L-cysteine + [enzyme]-cysteine Escherichia coli
-
 L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Escherichia coli
-
 selenide + L-alanine + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.7 Escherichia coli
-
-
-
4.4.1.16 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.1.7 L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.7 0.05
-
 H123 mutant with L-selenocysteine as substrate Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.7 L-cysteine + [enzyme]-cysteine
-
 Escherichia coli L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
2.8.1.7 L-cysteine + [enzyme]-cysteine Cys364 residue is essential for activity toward L-cysteine but not toward L-selenocyteine Escherichia coli L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
2.8.1.7 L-selenocysteine Cys364 residue is essential for activity toward L-cysteine but not toward L-selenocyteine Escherichia coli L-alanine + selenium
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Escherichia coli selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor enzyme bound pyridoxal 5'-phosphate serves as acceptor Escherichia coli selenide + L-alanine + acceptor
-
 ?
4.4.1.16 additional information traces of activity with L-cysteine Escherichia coli ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.1.7 pyridoxal 5'-phosphate
-
 Escherichia coli
4.4.1.16 pyridoxal 5'-phosphate required Escherichia coli