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Literature summary extracted from

  • Kambampati, R.; Lauhon, C.T.
    MnmA and IscS are required for in vitro 2-thiouridine biosynthesis in Escherichia coli (2003), Biochemistry, 42, 1109-1117.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.13
-
 Escherichia coli

General Stability

EC Number General Stability Organism
2.8.1.13 ATP protects MnmA from trypsinolysis Escherichia coli
2.8.1.13 tRNA(Glu) protects MnmA from tryptic digestion Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.7 L-cysteine + [enzyme]-cysteine Escherichia coli
-
 L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
2.8.1.7 additional information Escherichia coli involved in biosynthesis of 2-thiouridine ?
-
 ?
2.8.1.13 additional information Escherichia coli thionucleosides are uniquely present in tRNA. In many organisms, tRNAs specific for Lys, Glu, and Gln contain hypermodified 2-thiouridine (s2U) derivatives at wobble position 34. The s2 group of s2U34 stabilizes anticodon structure, confers ribosome binding ability to tRNA and improves reading frame maintenance ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.7 Escherichia coli P25745
-
-
2.8.1.13 Escherichia coli P25745
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.13
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.7 L-cysteine + [enzyme]-cysteine
-
 Escherichia coli L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
2.8.1.7 additional information involved in biosynthesis of 2-thiouridine Escherichia coli ?
-
 ?
2.8.1.13 additional information thionucleosides are uniquely present in tRNA. In many organisms, tRNAs specific for Lys, Glu, and Gln contain hypermodified 2-thiouridine (s2U) derivatives at wobble position 34. The s2 group of s2U34 stabilizes anticodon structure, confers ribosome binding ability to tRNA and improves reading frame maintenance Escherichia coli ?
-
 ?
2.8.1.13 additional information thionucleosides are uniquely present in tRNA. In many organisms, tRNAs specific for Lys, Glu, and Gln contain hypermodified 2-thiouridine (s2U) derivatives at wobble position 34. Purified IscS-persulfide is able to provide sulfur for in vitro s2U synthesis in the absence of cysteine. The s2 group of s2U34 stabilizes anticodon structure, confers ribosome binding ability to tRNA and improves reading frame maintenance. Identification of 2-thiouridine as product of the in vitro s2U reaction. Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.1.13 MnmA
-
 Escherichia coli