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Literature summary extracted from

  • Kiyasu, T.; Asakura, A.; Nagahashi, Y.; Hoshino, T.
    Contribution of cysteine desulfurase (NifS protein) to the biotin synthase reaction of Escherichia coli (2000), J. Bacteriol., 182, 2879-2885.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.1.6 NifS a member of the Nif protein family stimulates biotin production Escherichia coli
2.8.1.6 NifU a member of the Nif protein family stimulates biotin production Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.6
-
 Escherichia coli
2.8.1.7
-
 Escherichia coli
2.8.1.7
-
 Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.1.6 FeCl3 sulfur of the iron-sulfur cluster is provided by cysteine desulfurase EC 2.8.1.7 Escherichia coli
2.8.1.6 Iron the enzyme contains an iron-sulfur cluster Escherichia coli
2.8.1.7 Iron [Fe-S] cluster Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.7 140000
-
 NifU/NifS complex, gel filtration Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.7 additional information Escherichia coli enzyme contributes to the biotin synthase reaction, probably by supplying sulfur to the BioB protein ?
-
 ?
2.8.1.7 additional information Klebsiella pneumoniae enzyme participates in biotin synthase reaction, probably by supplying sulfur to the iron-sulfur cluster of biotin synthase ?
-
 ?
2.8.1.7 additional information Klebsiella pneumoniae M5a1 enzyme participates in biotin synthase reaction, probably by supplying sulfur to the iron-sulfur cluster of biotin synthase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.6 Escherichia coli
-
-
-
2.8.1.7 Escherichia coli
-
-
-
2.8.1.7 Klebsiella pneumoniae
-
 M5a1
-
2.8.1.7 Klebsiella pneumoniae M5a1
-
 M5a1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.6
-
 Escherichia coli
2.8.1.7
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.6 dethiobiotin + sulfur insertion of a sulfur atom between the inactive methyl and methylene carbon atoms adjacent to the imidazolinone ring Escherichia coli biotin
-
 ?
2.8.1.7 additional information enzyme contributes to the biotin synthase reaction, probably by supplying sulfur to the BioB protein Escherichia coli ?
-
 ?
2.8.1.7 additional information enzyme participates in biotin synthase reaction, probably by supplying sulfur to the iron-sulfur cluster of biotin synthase Klebsiella pneumoniae ?
-
 ?
2.8.1.7 additional information enzyme participates in biotin synthase reaction, probably by supplying sulfur to the iron-sulfur cluster of biotin synthase Klebsiella pneumoniae M5a1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.7 tetramer heterotetramer, NifU2NifS2 Klebsiella pneumoniae