Literature summary extracted from
Picciocchi, A.; Douce, R.; Alban, C.
Biochemical characterization of the Arabidopsis biotin synthase reaction. The importance of mitochondria in biotin synthesis (2001), Plant Physiol., 127, 1224-1233.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.8.1.6 |
D-fructose 1,6-bisphosphate |
enhances biotin formation |
Arabidopsis thaliana |
|
2.8.1.6 |
dithiothreitol |
required |
Arabidopsis thaliana |
|
2.8.1.6 |
L-cysteine |
highly stimulates |
Arabidopsis thaliana |
|
2.8.1.6 |
additional information |
one or more unidentified factors from mitochoncrial matrix of pea and potato and from mitochondrial membranes of pea, in addition to the purified enzyme, are obligatory for the conversion of dithiobiotin to biotin in plants |
Arabidopsis thaliana |
|
2.8.1.6 |
S-adenosyl-L-methionine |
required |
Arabidopsis thaliana |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.8.1.6 |
- |
Arabidopsis thaliana |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.8.1.6 |
acidomycin |
structural analog of biotin, complete inhibition at 0.4 mM, 50% inhibition at 0.035 mM |
Arabidopsis thaliana |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.8.1.6 |
0.03 |
- |
dethiobiotin |
pH 8, 37°C |
Arabidopsis thaliana |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.8.1.6 |
Fe2+ |
highly stimulates |
Arabidopsis thaliana |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.8.1.6 |
Arabidopsis thaliana |
- |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.8.1.6 |
dethiobiotin + sulfur |
- |
Arabidopsis thaliana |
biotin |
- |
? |
|
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
2.8.1.6 |
0.00055 |
- |
dethiobiotin |
pH 8, 37°C |
Arabidopsis thaliana |
|
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.8.1.6 |
NADH |
required |
Arabidopsis thaliana |
|
2.8.1.6 |
NADPH |
required |
Arabidopsis thaliana |
|