Literature summary extracted from

Ollagnier-de-Choudens, S.; Mulliez, E.; Hewitson, K.S.; Fontecave, M.
Biotin synthase is a pyridoxal phosphate-dependent cysteine desulfurase (2002), Biochemistry, 41, 9145-9152.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.8.1.6	dithiothreitol	10fold stimulation of cysteine desulfurase activity of the enzyme at 20 mM	Escherichia coli
2.8.1.6	L-cysteine	increases biotin production	Escherichia coli
2.8.1.6	pyridoxal 5'-phosphate	required for cysteine desulfurase activity of the enzyme	Escherichia coli

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.8.1.6	C128A	reduced cysteine desulfurase activity	Escherichia coli
2.8.1.6	C188A	cysteine desulfurase activity is reduced to 70% of the wild-type activity	Escherichia coli
2.8.1.6	C97A	reduced cysteine desulfurase activity	Escherichia coli

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.8.1.6	NaBH4	decreases cysteine desulfurase and biotin synthase activity	Escherichia coli

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.8.1.6	0.03	-	L-cysteine	pH 8, 37°C	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.8.1.6	Escherichia coli	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.8.1.6	dethiobiotin + sulfur	-	Escherichia coli	biotin	-	?
2.8.1.6	L-cysteine	enzyme displays cysteine desulfurase activity, providing it with the ability to mobilize sulfur from free cysteine	Escherichia coli	L-alanine + sulfide	-	?

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Release 2023.1 (January 2023)