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Literature summary extracted from

  • Kiyasu, T.; Asakura, A.; Nagahashi, Y.; Hoshino, T.
    Biotin synthase of Bacillus subtilis shows less reactivity than that of Escherichia coli in in vitro reaction systems (2002), Arch. Microbiol., 179, 26-32.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.1.6 S-adenosyl-L-methionine required Bacillus subtilis
2.8.1.6 S-adenosyl-L-methionine required Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.6
-
 Bacillus subtilis
2.8.1.6
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.1.6 Fe2+ enhances activity Bacillus subtilis
2.8.1.6 Fe2+ enhances activity Escherichia coli
2.8.1.6 Iron the enzyme contains an iron-sulfur cluster Bacillus subtilis
2.8.1.6 Iron the enzyme contains an iron-sulfur cluster Escherichia coli
2.8.1.6 S2- enhances activity Bacillus subtilis
2.8.1.6 S2- enhances activity Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.6 37000
-
 2 * 37000, SDS-PAGE Bacillus subtilis
2.8.1.6 80000
-
 gel filtration Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.6 Bacillus subtilis
-
-
-
2.8.1.6 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.6
-
 Bacillus subtilis
2.8.1.6
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.6 dethiobiotin + sulfur
-
 Bacillus subtilis biotin
-
 ?
2.8.1.6 dethiobiotin + sulfur
-
 Escherichia coli biotin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.6 dimer
-
 Escherichia coli
2.8.1.6 dimer 2 * 37000, SDS-PAGE Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.1.6 0.0000005
-
 dethiobiotin pH 7.5, 30°C, cell free extract Bacillus subtilis
2.8.1.6 0.0000142
-
 dethiobiotin pH 7.5, 30°C, cell free extract Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.1.6 NADPH
-
 Escherichia coli
2.8.1.6 NADPH required Bacillus subtilis