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Literature summary extracted from

  • Sanyal, I.; Cohen, G.; Flint, D.H.
    Biotin synthase: purification, characterization as a [2Fe-2S]cluster protein, and in vitro activity of the Escherichia coli bioB gene product (1994), Biochemistry, 33, 3625-3631.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.6 0.002
-
 dethiobiotin pH 7.5 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.1.6 Iron the 104000 Da dimeric enzyme form contains a single [2Fe-2S] cluster per dimer Escherichia coli
2.8.1.6 Iron the 82000 Da dimeric enzyme form contains one [2Fe-2S]cluster per monomer Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.6 38700
-
 2 * 38700, calculation from nucleotide sequence Escherichia coli
2.8.1.6 39000
-
 2 * 39000, 82000 MW form and 104000 MW form, SDS-PAGE Escherichia coli
2.8.1.6 82000
-
 gel electrophoresis Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.6 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.6
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.6 dethiobiotin + sulfur
-
 Escherichia coli biotin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.6 dimer 2 * 38700, calculation from nucleotide sequence Escherichia coli
2.8.1.6 dimer 2 * 39000, 82000 MW form and 104000 MW form, SDS-PAGE Escherichia coli