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Literature summary extracted from

  • Bui, T.S.; Marquet, A.
    Biotin synthase of Bacillus sphaericus (1997), Methods Enzymol., 279, 356-362.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.1.6 dithiothreitol
-
 Lysinibacillus sphaericus
2.8.1.6 L-cysteine
-
 Lysinibacillus sphaericus
2.8.1.6 S-adenosyl-L-methionine absolute requirement as electron source Lysinibacillus sphaericus

General Stability

EC Number General Stability Organism
2.8.1.6 loses all its clusters if the purification is carried out with buffers that have not been saturated with argon Lysinibacillus sphaericus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.6 Fe2+ above 1 mM Lysinibacillus sphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.6 0.0016
-
 dethiobiotin
-
 Lysinibacillus sphaericus
2.8.1.6 0.005
-
 9-Mercaptodethiobiotin
-
 Lysinibacillus sphaericus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.1.6 Iron presence of a [2Fe-2S] cluster Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.6 36954
-
 2 * 36954, calculation from nucleotide sequence Lysinibacillus sphaericus
2.8.1.6 37000
-
 2 * 37000, SDS-PAGE Lysinibacillus sphaericus
2.8.1.6 75000
-
 gel filtration Lysinibacillus sphaericus

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.6 Lysinibacillus sphaericus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.8.1.6 oxygen sensitive Lysinibacillus sphaericus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.6
-
 Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.6 additional information
-
-
 Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.6 9-mercaptodethiobiotin + sulfur
-
 Lysinibacillus sphaericus biotin
-
 ?
2.8.1.6 dethiobiotin + sulfur ultimate sulfur donor has not yet been identified Lysinibacillus sphaericus biotin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.6 dimer 2 * 37000, SDS-PAGE Lysinibacillus sphaericus
2.8.1.6 dimer 2 * 36954, calculation from nucleotide sequence Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.1.6 NADPH
-
 Lysinibacillus sphaericus