EC Number | Cloned (Comment) | Organism |
---|---|---|
2.8.1.2 | - |
Leishmania mexicana |
2.8.1.2 | wild-type and 4 truncated versions lacking 10, 32, 50 and 70 amino acids at the C-terminus | Leishmania major |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.1.2 | 0.2 | - |
3-Mercaptopyruvate | pH 7.3, 37°C | Leishmania major | |
2.8.1.2 | 0.3 | - |
reduced thioredoxin | pH 7.3, 37°C | Leishmania major | |
2.8.1.2 | 1.7 | - |
thiosulfate | pH 7.3, 37°C | Leishmania major |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.8.1.2 | cytosol | - |
Leishmania major | 5829 | - |
2.8.1.2 | cytosol | - |
Leishmania mexicana | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.1.2 | 40000 | - |
SDS-PAGE | Leishmania major |
2.8.1.2 | 40000 | - |
SDS-PAGE | Leishmania mexicana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.1.2 | 3-mercaptopyruvate + cyanide | Leishmania major | - |
pyruvate + thiocyanate | - |
? | |
2.8.1.2 | 3-mercaptopyruvate + cyanide | Leishmania mexicana | - |
pyruvate + thiocyanate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.1.2 | Leishmania major | Q7K9G0 | - |
- |
2.8.1.2 | Leishmania mexicana | Q8T381 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.1.2 | - |
Leishmania mexicana |
2.8.1.2 | wild-type and 4 truncated versions lacking 10, 32, 50 and 70 amino acids at the C-terminus | Leishmania major |
EC Number | Renatured (Comment) | Organism |
---|---|---|
2.8.1.2 | enzyme regains full activity without the need for assistance in the form of a chaperone or detergent after denaturation is 6 M urea and renaturation using dialysis or dilution | Leishmania major |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.8.1.2 | amastigote | - |
Leishmania major | - |
2.8.1.2 | promastigote | - |
Leishmania major | - |
2.8.1.2 | promastigote | - |
Leishmania mexicana | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.8.1.2 | -20°C, stable for at least 12 months without loss of activity | Leishmania major |
2.8.1.2 | -20°C, stable for at least 12 months without loss of activity | Leishmania mexicana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.1.2 | 3-mercaptopyruvate + cyanide | - |
Leishmania major | pyruvate + thiocyanate | - |
? | |
2.8.1.2 | 3-mercaptopyruvate + cyanide | - |
Leishmania mexicana | pyruvate + thiocyanate | - |
? | |
2.8.1.2 | sulfane sulfur + cyanide | - |
Leishmania major | thiocyanate + ? | - |
? | |
2.8.1.2 | sulfane sulfur + cyanide | - |
Leishmania mexicana | thiocyanate + ? | - |
? | |
2.8.1.2 | thiosulfate + thioredoxin | - |
Leishmania major | ? | - |
? | |
2.8.1.2 | thiosulfate + thioredoxin | - |
Leishmania mexicana | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.1.2 | 6.9 | 7.6 | - |
Leishmania major |
2.8.1.2 | 6.9 | 7.6 | - |
Leishmania mexicana |