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Literature summary extracted from

  • Magahara, N.; Okazaki, T.; Nishino, T.
    Cytosolic mercaptopyruvate sulfurtransferase is evolutionarily related to mitochondrial rhodanese. Striking similarity in active site amino acid sequence and the increase in the mercaptopyruvate sulfurtransferase activity of rhodanese by site-directed mutagenesis (1995), J. Biol. Chem., 270, 16230-16235.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.2 73
-
 3-Mercaptopyruvate pH 9.6 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.1.2 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.2 34000
-
 1 * 34000, enzyme exists as a monomer and homodimer, SDS-PAGE, gel filtration Rattus norvegicus
2.8.1.2 34000
-
 2 * 34000, enzyme exists as a monomer and homodimer, SDS-PAGE, gel filtration Rattus norvegicus
2.8.1.2 34500
-
 two peaks with molecular masses of 34500 Da and 53500 Da, enzyme exists as a monomer and homodimer, gel filtration Rattus norvegicus
2.8.1.2 53000
-
 two peaks with molecular masses of 34500 Da and 53500 Da, enzyme exists as a monomer and homodimer, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.2 3-mercaptopyruvate + cyanide Rattus norvegicus
-
 pyruvate + thiocyanate
-
 ?
2.8.1.2 3-mercaptopyruvate + cyanide Rattus norvegicus Wistar
-
 pyruvate + thiocyanate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.2 Rattus norvegicus
-
 Wistar
-
2.8.1.2 Rattus norvegicus Wistar
-
 Wistar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.2 homogeneity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.1.2 liver predominantly in pericentral hepatocytes Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.2 3793
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.2 3-mercaptopyruvate + cyanide
-
 Rattus norvegicus pyruvate + thiocyanate
-
 ?
2.8.1.2 3-mercaptopyruvate + cyanide
-
 Rattus norvegicus Wistar pyruvate + thiocyanate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.2 dimer 2 * 34000, enzyme exists as a monomer and homodimer, SDS-PAGE, gel filtration Rattus norvegicus
2.8.1.2 monomer 1 * 34000, enzyme exists as a monomer and homodimer, SDS-PAGE, gel filtration Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.1.2 25
-
 3-Mercaptopyruvate pH 9.55 Rattus norvegicus