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Literature summary extracted from

  • Magahara, N.; Ito, T.; Minami, M.
    Mercaptopyruvate sulfurtransferase as a defense against cyanide toxication: molecular properties and mode of detoxification (1999), Histol. Histopathol., 14, 1277-1286.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.2 liver enzyme Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.2 R196G decrease in kcat/Km Rattus norvegicus
2.8.1.2 S249K decrease in kcat/Km Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.1.2 cytoplasm
-
 Rattus norvegicus 5737
-
2.8.1.2 mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.2 3-mercaptopyruvate + cyanide Rattus norvegicus
-
 pyruvate + thiocyanate
-
 ?
2.8.1.2 additional information Rattus norvegicus cyanide detoxification ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.2 homogeneity Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.1.2 3-mercaptopyruvate + reduced thioredoxin = pyruvate + hydrogen sulfide + oxidized thioredoxin mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.1.2 brain
-
 Rattus norvegicus
-
2.8.1.2 cerebellum
-
 Rattus norvegicus
-
2.8.1.2 cerebrum
-
 Rattus norvegicus
-
2.8.1.2 glial cell
-
 Rattus norvegicus
-
2.8.1.2 heart
-
 Rattus norvegicus
-
2.8.1.2 kidney predominantly in tubular epithelium Rattus norvegicus
-
2.8.1.2 liver predominantly in pericentral hepatocytes Rattus norvegicus
-
2.8.1.2 lung
-
 Rattus norvegicus
-
2.8.1.2 testis
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.2 3-mercaptopyruvate + cyanide
-
 Rattus norvegicus pyruvate + thiocyanate
-
 ?
2.8.1.2 additional information cyanide detoxification Rattus norvegicus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.1.2 0.0783
-
 3-Mercaptopyruvate S249K mutant Rattus norvegicus
2.8.1.2 0.233
-
 3-Mercaptopyruvate G248R mutant Rattus norvegicus
2.8.1.2 0.917
-
 3-Mercaptopyruvate S249A mutant Rattus norvegicus
2.8.1.2 1.03
-
 3-Mercaptopyruvate wild-type enzyme Rattus norvegicus
2.8.1.2 1.07
-
 3-Mercaptopyruvate R196G mutant Rattus norvegicus
2.8.1.2 5.67
-
 3-Mercaptopyruvate R187G mutant Rattus norvegicus